The aim of this study was to design a resolution typing system for the HLA-B gene. This technique involves a one-step PCR reaction utilizing genomic DNA and sequence-specific primers to determine the specificity of each allele and to produce a larger primer data base ideal for serological analysis. The application of this technique to serological analysis can improve serology detection which is currently hindered by antibody cross-reactivity and the unavailability of useful typing reagents.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc278947 |
| Date | 08 1900 |
| Creators | Chiu, Angela Chen-Yen |
| Contributors | Shanley, Mark Stephen, Benjamin, Robert C., O'Donovan, Gerard A. |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | viii, 74 leaves: ill., Text |
| Rights | Public, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved., Chiu, Angela Chen-Yen |
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