Thesis (MSc (Biochemistry))--University of Stellenbosch, 2009. / ENGLISH ABSTRACT:
This study describes:
a) the isolation and purification of horseradish peroxidase isoenzymes from horseradish roots,
b) the characterization of various forms and components of the enzyme by cation-exchange and
reversed-phase high performance liquid chromatography,
c) the preparation of antibodies against horseradish peroxidase isoenzymes,
d) immunological studies for the development of an isoenzyme quantification method and
e) the formation of an enzyme-melamine conjugate for use in a melamine quantification
immunoassay. / AFRIKAANSE OPSOMMING:
Hierdie studie beskryf:
a) die isolering en suiwering van peperwortel-peroksidase-isoënsieme vanuit die peperwortel,
b) die karakterisering van verskillende vorme en komponente van dié ensiem deur katioonuitruilings
en omgekeerde-fase HPLC
c) die voorbereiding van teenliggaampies vir peperwortel-peroksidase-isoënsieme,
d) immunologiese studies vir die ontwikkeling van 'n isoënsiem-kwantifiseringsmetode; en
e) die vorming van 'n ensiem-melamien-konjugaat vir gebruik in 'n melamienkwantifiseringsmetode.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/1587 |
| Date | 12 1900 |
| Creators | Odendaal, Ruenda |
| Contributors | Swart, P., Lombard, N., University of Stellenbosch. Faculty of Science. Dept. of Biochemistry. |
| Publisher | Stellenbosch : University of Stellenbosch |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Rights | University of Stellenbosch |
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