Influence of media age, initial dark period, inoculation with Agrobacterium, and leaf maturity on the regeneration and genetic transformation of two strawberry cultivars was investigated. Four cultivars of blackberry and one raspberry were included to test their response to media age and dark period. Leaf strips of Chandler and Cardinal strawberry, blackberry, and raspberry were exposed to a four-week dark treatment on modified MS media and subcultured to fresh media. One group of leaf strips was left on the original media for the entire eight weeks. Another group of explants, along with the media treatments was given an additional four-week dark period.
Chandler strawberry leaf strips exposed to four weeks dark and subcultured onto fresh media produced 19% more plantlets than Chandler exposed to eight weeks dark. Chandler given a four-week dark period and subcultured onto fresh media produced 58% more plantlets than those left on their original media. Chandler produced 97% more plantlets than Cardinal strawberry produced with any treatment. Cardinal strawberry, blackberry and raspberry responded by producing copious amounts of callus.
When inoculated with Agrobacterium, the highest regeneration rate for Chandler or Cardinal strawberry leaf strips was less than 0.2 plantlets per leaf strip. This was a 96% reduction in regeneration rate compared to the previous experiment in which Agrobacterium was not introduced.
In an experiment to test the effect of leaf maturity on regeneration rate, 59% more plantlets were produced from unexpanded strawberry leaf strips than leaf strips from newly expanded or mature leaves. Unexpanded Chandler strawberry leaf strips, exposed to a four-week dark period followed by subculturing onto fresh media, have a higher regeneration rate than those given an eight-week dark period or left on their original media. Chandler strawberry leaf strips from unexpanded leaves have a higher regeneration rate than those from newly expanded or mature leaves.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-11032004-104040 |
| Date | 08 November 2004 |
| Creators | Whitley, Kristi Lee |
| Contributors | Charles E. Johnson, Don Labonte, Jeff Kuehny |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-11032004-104040/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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