In our previous studies, ingestion of prebiotics in low fat diets resulted in decreased cecal pH from 8 to about 6 and increases in short chain fatty acids indicating robust fermentation. However, in some preliminary studies this effect on fermentation was not seen when rodents were obese and/or fed a high-fat diet. This comprehensive high-fat diet study was conducted to determine which sources and combinations of prebiotics would enhance fermentation despite a high dietary fat content. The effects of prebiotics in a high-fat diet (44% energy) on pH, cecal weights, abdominal fat, and body weight were studied in mature male C57Bl/6 mice fed one of 9 diets of similar energy (4.0 ± 0.2 kcal/g) for 12 weeks. A control (C) diet was compared to 4 prebiotics: Hi-Maize® RS2 (R), Novelose® RS3 (N), Nutraflora® fructooligosaccharide (F), BENEO-Orafti HP gel® inulin (I), which were fed individually and combined (F+I, R+F, R+I, and R+N). Results were significant at p<0.05. Fermentation, indicated by lower pH values, occurred with all F and I diets and combination diets. However, none of the groups had reduced abdominal fat compared to control as has been observed in previous studies with consumption of RS in low fat diets. All prebiotic diets had larger empty ceca, but only F and I had greater full ceca than the RS groups. It is proposed that diets with high concentrations of fat affect monogastric fermentation and microbial populations in a manner similar to ruminants. It is possible that the beneficial health effects of prebiotic ingestion may be most effective if consumed with a low-fat diet.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-11182010-093506 |
| Date | 19 November 2010 |
| Creators | Goldsmith, Felicia Robin |
| Contributors | Martin, Roy, Finley, John W., Keenan, Michael |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-11182010-093506/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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