Membrane technology has been actively used as a separation tool in the chemical, environmental, and biopharmaceutical industries for several decades. As membrane quality requirement in the industry has increased, efforts have been directed towards enhancement in mechanical strength, chemical durability and functionality of membranes. One of the approaches for membrane quality enhancement is based on the combination of hydrogel technology with membrane technology. This thesis focused on the application and development of hydrogel based membranes, notably hydrophilized PVDF (polyvinylidene fluoride) membrane for hydrophobic interaction membrane chromatography; the fabrication of paper-hydrogel composite membranes for membrane chromatography; development of a technique for coating alginate (a natural hydrogel) on the outer surface of a hollow fiber membrane for potential application in bioreactors and the use of hollow fiber membranes as mold for fabrication calcium alginate fibers for biomedical and tissue engineering applications.
A membrane chromatography-based polishing technique was developed for removing leached protein-A and aggregates from monoclonal antibody (mAb). A commercial synthetic membrane that is known to be hydrophilized by hydrogel grafting was employed to develop this polishing process that resulted in highly pure mAb, free from aggregates and protein-A. This mAb polishing technique could easily be integrated with a hydrophobic interaction membrane chromatography based mAb purification process.
A paper-hydrogel composite membrane was developed as an inexpensive alternative to commercial synthetic membranes used for carrying hydrophobic interaction membrane chromatography. Poly(N-vinylcaprolactam) or PVCL hydrogel was coated on Whatman filter paper to prepare these membranes. These environment responsive membrane which responded to changes in salt concentration, gave excellent fractionation of multi-component protein mixtures. As case study, a mixture of immunoglobulin G, human serum albumin and insulin was fractionated.
A technique for modifying the surface of synthetic hollow fiber membranes with alginate (a natural hydrogel) was developed. This manner of surface modification led to the improvement in membrane mass transport. The alginate was cross-linked on the outer surface of the membrane by diffusion of the cross-linker (calcium ions) through the membrane pores. The calcium alginate coating layer was characterized by optical and transmission electron microscopy, contact angle measurement, hydraulic permeability measurement and by examining solute transport.
Hollow and solid calcium alginate fibers were fabricated using a novel hollow fiber membrane based moulding technique. The pore present on the hollow fiber membrane served as the reservoir for the calcium chloride solution with cross-linked the alginate within the lumen. The calcium alginate fibers produced were characterized by optical, transmission electron, and scanning electron microscopy. Cell immobilization experiments were carried out to demonstrate biocompatibility and potential for tissue engineering applications. / Thesis / Doctor of Philosophy (PhD)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/15406 |
| Date | January 2014 |
| Creators | YOO, SEUNG MI |
| Contributors | GHOSH, RAJA, Chemical Engineering |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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