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Kvasinky kolonizující povrchy listů a jejich identifikace / Yeasts colonizing the leaf surfaces and their identification

This diploma thesis is focused on optimalization and employing the PCR-RFLP method, based on the molecular biology principles, for an identification and taxonomy of the yeasts which colonize the leaf surfaces. Simultaneously the yeasts identification techniques based on physiological and morfphological attributes are compared and replaced. PCR-RFLP takes advantage of thermostable polymerases´ ability to amplify the specific segment in the rDNA, which can be split by restriction endonucleases to characteristical polymorphical fragments. Comparing these fragments and restriction´s positions which are for each species unique, demanded results were obtained. They´re summarized in the conclusion part. The theoretical part describes the morphology and cytology of the yeasts, taxonomy as a science, genuses of examined yeasts Cryptococcus, Rhodotorula a Saccharomyces are covered more thoroughly and the method of PCR-RFLP is described in detail.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:216579
Date January 2010
CreatorsBělochová, Kamila
ContributorsVojtíšková, Marie, Vránová, Dana
PublisherVysoké učení technické v Brně. Fakulta chemická
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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