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Immunochemical characterisation of plasma immunoglobulins in IgA nephropathy.

Chui Shiu Hon. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 144-183. / ACKNOWLEDGEMENTS / SUMMARY / LIST OF ABBREVIATIONS / Chapter CHAPTER 1. --- INTRODUCTION --- p.1 / Chapter 1.1 --- IgA Nephropathy --- p.1 / Chapter 1.1.1 --- History & Epidemiology --- p.1 / Chapter 1.1.2 --- Clinical Pathological Features --- p.2 / Chapter 1.1.3 --- Laboratory Findings --- p.3 / Chapter 1.1.4 --- Immunopathogenesis --- p.5 / Chapter 1.2 --- The Role of IgA in the Pathogenesis of IgA Nephropathy --- p.10 / Chapter 1.2.1 --- Structure of IgA Molecule --- p.10 / Chapter 1.2.2 --- IgA Biosynthesis and Immune Regulation --- p.23 / Chapter 1.2.3 --- Biological Role of IgA --- p.26 / Chapter 1.2.4 --- Circulating Immune Complexes in IgA Nephropathy --- p.27 / Chapter 1.2.5 --- IgA Subclasses in IgA Nephropathy --- p.32 / Chapter 1.2.6 --- Light Chain Composition of IgA in IgA Nephropathy --- p.33 / Chapter 1.3 --- Aim of the Present Study --- p.36 / Chapter 1.4 --- Design of Experiments --- p.37 / Chapter CHAPTER 2. --- MATERIALS & METHODS --- p.42 / Chapter 2.1 --- Materials --- p.42 / Chapter 2.1.1 --- Patients --- p.42 / Chapter 2.1.2 --- Controls --- p.43 / Chapter 2.1.3 --- Additional Specimens --- p.43 / Chapter 2.1.4 --- Serum Samples --- p.44 / Chapter 2.1.5 --- Chemicals --- p.44 / Chapter 2.1.6 --- Immunoglobulins --- p.46 / Chapter 2.1.7 --- Antisera --- p.47 / Chapter 2.1.8 --- Solutions and Buffers --- p.48 / Chapter 2.1.9 --- Apparatus and Equipment --- p.51 / Chapter 2.2 --- Methods --- p.53 / Chapter 2.2.1 --- Serum Protein Electrophoresis --- p.53 / Chapter 2.2.2 --- Immunochemical Techniques --- p.54 / Chapter 2.2.3 --- Fast Protein Liquid Chromatography (FPLC) for Isolation of Serum IgA --- p.64 / Chapter CHAPTER 3. --- SERUM PROTEIN ELECTROPHORESIS AND IMMUNOGLOBULIN CONCENTRATIONS --- p.67 / Chapter 3.1 --- Serum Protein Electrophoresis --- p.67 / Chapter 3.2 --- Serum Immunoglobulin Concentration --- p.67 / Chapter 3.3 --- Discussion --- p.70 / Chapter CHAPTER 4. --- LIGHT CHAIN RATIOS OF INDIVIDUAL IMMUNOGLOBULINS --- p.72 / Chapter 4.1 --- Enzyme-Linked Immunosorbent Assay for Light Chain Concentrations --- p.72 / Chapter 4.2 --- Light Chain Ratios of Individual Serum Immunoglobulins in Normal Subjects and in Disease --- p.75 / Chapter 4.3 --- Discussion --- p.77 / Chapter CHAPTER 5. --- IN VITRO SYNTHESIS OF IgA WITH LAMBDA LIGHT CHAIN IN IgA NEPHROPATHY --- p.83 / Chapter 5.1 --- Lymphocyte Culture and In Vitro Immunoglobulin Production --- p.83 / Chapter 5.2 --- In Vitro Immunoglobulin Production and Predominant Synthesis of IgA with λ Light Chain in IgA Nephropathy --- p.83 / Chapter 5.3 --- Dicussion --- p.87 / Chapter CHAPTER 6. --- PURIFICATION OF SERUM IgA BY AFFINITY CHROMATOGRAPHY --- p.90 / Chapter 6.1 --- Fast Protein Liquid Chromatography --- p.90 / Chapter 6.2 --- Recovery of Isolated IgA --- p.92 / Chapter 6.3 --- Purity of Isolated IgA --- p.92 / Chapter 6.4 --- κ/λ Ratio of IgA Before and After FPLC --- p.96 / Chapter 6.5 --- Subclass of IgA Before and After FPLC --- p.97 / Chapter 6.6 --- Discussion --- p.100 / Chapter CHAPTER 7. --- "ANALYSIS OF CHARGE DISTRIBUTION OF IgA, IgA(κ) AND IgA(λ)" --- p.102 / Chapter 7.1 --- "Iso-Electric Focusing, Immunoblotting, and Densitometry of Purified IgA for Total IgA, IgA(κ) and IgA (λ)" --- p.102 / Chapter 7.2 --- "Charge Distribution of Plasma Total IgA, IgA(κ) and IgA(λ)" --- p.103 / Chapter 7.2.1 --- A/C Ratio of Total IgA --- p.103 / Chapter 7.2.2 --- A/C Ratio of IgA(κ) --- p.113 / Chapter 7.2.3 --- A/C Ratio of IgA(λ) --- p.113 / Chapter 7.3 --- Discussion --- p.129 / Chapter CHAPTER 8. --- GENERAL DISCUSSION --- p.134 / REFERENCES --- p.140 / APPENDICES

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318637
Date January 1990
ContributorsChui, Shiu Hon., Chinese University of Hong Kong Graduate School. Division of Clinical and Pathological Sciences.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, 6, 183, [37] leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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