The effect of culture media and mycotoxin on porcine preimplantation embryonic development has been investigated. In the first experiment, porcine embryonic cleavage rates were similar in NCSU-23 and PZM-3 culture media, while more blastocysts were produced in PZM-3 (P<0.05). BAX and BCL2L1 transcription levels were similar in blastocysts cultured in both media. Cleavage rates were significantly decreased in the presence of cycloheximide (P<0.05), and both á-amanitin and cycloheximide completely inhibited blastocyst formation. In the mycotoxin experiment, porcine embryonic cleavage rates decreased in 10 ìM á-ZEA group, while blastocyst rates decreased in 30 ìM á-ZEA group (P<0.05). Total cell numbers of blastocysts were significantly lower in the 10 µM á-ZEA group (P<0.05). The transcription levels of POU5F1 and BCL2L1 were similar, while that of BAX and the ratio of BAX/BCL2L1 significantly increased in 3 ìM and 10 ìM á-ZEA groups compared to control group.
| Identifer | oai:union.ndltd.org:MSSTATE/oai:scholarsjunction.msstate.edu:td-4569 |
| Date | 09 August 2008 |
| Creators | Wang, Hongfeng |
| Publisher | Scholars Junction |
| Source Sets | Mississippi State University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Theses and Dissertations |
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