Burns often leads to infection, due to damage to the skin's protective barrier. Burn injury has been repeatedly shown to induce considerable inflammatory and immune dysfunction. The innate immune system is a universal and ancient form of host defense against infection. Activation of innate immunity constitutes the first line of host defense against infection. Neutrophils are white blood cells and part of the immune system. They are the most common PMN (polymorphonuclear neutrophils) and accounted for 70% of all leukocytes. Neutrophils provided the first line of defense of the innate immune system by phagocytosing, killing, and digesting bacteria and fungi. Priming means a process whereby the response of neutrophils to an activating stimulus is potentiated, sometimes greatly, by prior to exposure to priming agents such as tumor necrosis factor-alpha(TNF-alpha), platelet-activating factor (PAF). Neutrophil priming causes a dramatic increase in the response of these cells to an activating agent; this process has been shown to be critical for neutrophil-mediated tissue injury both in vitro and in vivo. However, the intracellular signaling pathways used by neutrophil in response to pro-inflammatory stimuli have not been elucidated. The discovery of TLRs has made us understanding of the mechanisms of innate immune recognition. The innate immune system detectes the invasion of microorganism through TLRs, which recognize microbial components and trigger inflammatory responses. Severe burn injury produces shock and induces acute gastrointestinal derangement that may disrupt gastrointestinal mucosa integrity and facilitate the bacterial translocation (BT) to Mesenteric lymph node (MLN), liver, and spleen. Hypertonic saline (HTS) has been advocated in thermal injury resuscitation because of the possibility of giving less total volume of resuscitation fluid, with a resulting decrease in edema and the need for escharotomy. In this study, I found that priming effect of BM neutrophils is TNF-alpha and p38 dependent and TLRs play a critical role to the innate immunity by recognizing bacteria and HTS enhance host response to bacterial challenge by increasing TLRs of inflammatory cells.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0826105-184432 |
| Date | 26 August 2005 |
| Creators | Huang, Hau-lun |
| Contributors | Long-sen Chang, Lee-wei Chen, Ching-mei Hsu, Pei-jung Lu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0826105-184432 |
| Rights | off_campus_withheld, Copyright information available at source archive |
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