Poon, Shui-Kei. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 78-98). / Abstracts in English and Chinese. / Abstract (in English) --- p.ii / Abstract (in Chinese) --- p.iv / Acknowledgement --- p.vi / Table of content --- p.vii / List of figures --- p.x / Symbols and abbreviations --- p.xii / Chapter 1 General Introduction / Chapter 1.1 --- Structure of ovarian follicles --- p.1 / Chapter 1.2 --- Folliculogenesis and its control --- p.1 / Chapter 1.2.1 --- History of inhibin discovery --- p.4 / Chapter 1.2.2 --- Inhibin and its related proteins --- p.5 / Chapter 1.3 --- Inhibin --- p.6 / Chapter 1.3.1 --- Structure --- p.6 / Chapter 1.3.2 --- Function --- p.7 / Chapter 1.3.3 --- Signaling --- p.11 / Chapter 1.3.4 --- Expression --- p.14 / Chapter 1.3.5 --- Regulation --- p.15 / Chapter 1.4 --- Objectives of the present study --- p.19 / Chapter Chapter 2 --- Spatiotemperoal Expression Profiles of Inhibin a in the Zebrafish Ovary / Chapter 2.1 --- Introduction --- p.25 / Chapter 2.2 --- Materials and Methods --- p.27 / Chapter 2.2.1 --- Animals --- p.27 / Chapter 2.2.2 --- Chemicals --- p.27 / Chapter 2.2.3 --- Separation of oocytes and follicular layers --- p.28 / Chapter 2.2.4 --- Isolation of ovarian follicles --- p.28 / Chapter 2.2.5 --- RNA isolation and reverse transcription --- p.28 / Chapter 2.2.6 --- Real-time and semi-quantitative RT-PCR quantification of expression --- p.29 / Chapter 2.2.4 --- Data analysis --- p.30 / Chapter 2.3 --- Results --- p.30 / Chapter 2.3.1 --- Validation of semi-quantitative RT-PCR quantification --- p.30 / Chapter 2.3.2 --- Tissue distribution of inha expression --- p.30 / Chapter 2.3.3 --- Localization of inha expression within the ovarian follicle --- p.31 / Chapter 2.3.4 --- Further evidence for inha expression in the follicle layer --- p.31 / Chapter 2.3.5 --- Stage-dependent expression of inha in the ovarian follicles --- p.32 / Chapter 2.4 --- Discussion --- p.33 / Chapter Chapter 3 --- Regulation of Inhibin a Expression in vitro and in vivo and the Effect of Inhibin on Final Oocyte Maturation / Chapter 3.1 --- Introduction --- p.43 / Chapter 3.2 --- Materials and Methods --- p.46 / Chapter 3.2.1 --- Animals --- p.46 / Chapter 3.2.2 --- Chemicals and hormones --- p.46 / Chapter 3.2.3 --- Preparation of goldfish pituitary extract --- p.47 / Chapter 3.2.4 --- Ovarian fragment incubation --- p.47 / Chapter 3.2.5 --- Preparation of spontaneously matured follicles --- p.48 / Chapter 3.2.6 --- Intra-peritoneal injection --- p.48 / Chapter 3.2.7 --- RNA isolation and reverse transcription --- p.48 / Chapter 3.2.8 --- Real-time and semi-quantitative RT-PCR quantification of expression --- p.48 / Chapter 3.2.9 --- Data analysis --- p.49 / Chapter 3.3 --- Results --- p.49 / Chapter 3.3.1 --- Validation of semi-quantitative RT-PCR quantification --- p.49 / Chapter 3.3.2 --- Temporal change of basal inha expression in cultured ovarian fragments --- p.49 / Chapter 3.3.3 --- Effect of pituitary extract on the expression of inha --- p.50 / Chapter 3.3.4 --- Effects of recombinant zebrafish FSH and LH on inha expression --- p.51 / Chapter 3.3.5 --- Effect of forskolin on inha expression --- p.52 / Chapter 3.3.6 --- Involvement of PKA and p38MAPK in the pituitary extract and forskolin-induced up-regulation of inha expression --- p.52 / Chapter 3.3.7 --- Effect of recombinant zfFSH on the expression of inha in vivo --- p.53 / Chapter 3.3.8 --- Effects of inhibin on the basal and DHP-induced final oocyte maturation --- p.53 / Chapter 3.4 --- Discussion --- p.54 / Chapter Chapter 4 --- General Discussion --- p.71 / References --- p.78
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326136 |
| Date | January 2007 |
| Contributors | Poon, Shui-kei., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xiii, 98 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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