Return to search

Interaction Studies of Secreted Aspartic Proteases (Saps) from <i>Candida albicans</i> : Application for Drug Discovery

<p>This thesis is focused on enzymatic studies of the secreted aspartic proteases (Saps) from <i>Candida albicans</i> as a tool for discovery of anti-<i>candida</i> drugs. <i>C. albicans</i> causes infections in a number of different locations, which differ widely in the protein substrates available and pH. Since <i>C. albicans</i> needs Saps during virulent growth, these enzymes are good targets for drug development.</p><p>In order to investigate the catalytic characteristics of Saps and their inhibitor affinities, substrate-based kinetic assays were developed. Due to the low sensitivity of these assays, especially at the sub-optimal pH required to mimic the different locations of infections, these assays were not satisfactory. Therefore, a biosensor assay was developed whereby, it was possible to study interaction between Saps and inhibitors without the need to optimise catalytic efficacy. Furthermore, the biosensor assay allowed determination of affinity, as well as the individual association and dissociation rates for inhibitor interactions.</p><p>Knowledge about substrate specificity, Sap subsite adaptivity, and the pH dependencies of catalytic efficacy has been accumulated. Also, screening of transition-state analogue inhibitors designed for HIV-1 protease has revealed inhibitors with affinity for Saps. Furthermore, the kinetics and pH dependencies of their interaction with Saps have been investigated. One of these inhibitors, BEA-440, displayed a complex interaction with Saps, indicating a conformational change upon binding and a very slow dissociation rate. A time dependent interaction was further supported by inhibition measurements. The structural information obtained affords possibilities for design of new more potent inhibitors that might ultimately become drugs against candidiasis. The strategy to combine substrate specificity studies with inhibitor screening has led to complementary results that generate a framework for further development of potent inhibitors.</p>

Identiferoai:union.ndltd.org:UPSALLA/oai:DiVA.org:uu-5815
Date January 2005
CreatorsBackman, Dan
PublisherUppsala University, Department of Biochemistry, Uppsala : Acta Universitatis Upsaliensis
Source SetsDiVA Archive at Upsalla University
LanguageEnglish
Detected LanguageEnglish
TypeDoctoral thesis, comprehensive summary, text
RelationDigital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, 1651-6214 ; 58

Page generated in 0.002 seconds