Marine sponges interact and coexist with many different organisms. A two-sponge
association between Amphimedon erina and Geodia gibberosa commonly occurs in the
Florida Keys. Previous studies have only focused on the ecological influence of the
association; they did not examine the cellular basis of the association. This association
between A. erina and G. gibberosa was used in the development of an in vitro model to
further the understanding of the cellular basis of natural sponge-sponge associations. In
this study, sponge cells were cultured individually and in co-cultures and their responses
related to apoptosis, cell death, and proliferation were monitored using high content
imaging. Co-cultured cells of species that form sponge-sponge associations did not have
the same cellular responses compared to co-cultured cells of species that do not form
sponge-sponge associations. Protein expression analyses demonstrated that the model that
was established does not mimic the cellular response of the association in nature, but this
model can be used to test in vitro cellular interactions of sponge species that do not form
associations in nature. In addition, the protein expression data that were obtained revealed that sponges use similar apoptotic pathways as humans and suggest that sponge cells may
shut down cell cycling in order to repair damaged DNA. This research is a small piece to
the puzzle that is sponge cell culture research. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2017. / FAU Electronic Theses and Dissertations Collection
| Identifer | oai:union.ndltd.org:fau.edu/oai:fau.digital.flvc.org:fau_34537 |
| Contributors | Conkling, Megan (author), Pomponi, Shirley A. (Thesis advisor), Florida Atlantic University (Degree grantor), Charles E. Schmidt College of Science, Department of Biological Sciences |
| Publisher | Florida Atlantic University |
| Source Sets | Florida Atlantic University |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation, Text |
| Format | 66 p., application/pdf |
| Rights | Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder., http://rightsstatements.org/vocab/InC/1.0/ |
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