<p>Advances in mass spectrometry (MS)
instrumentation and techniques have provided approaches for complementing
current biochemical research. Native
mass spectrometry, which aims to analyze intact biomolecules and biomolecular
complexes, has become a powerful tool for identifying and measuring different
units of complex structures as well as probing interactions among the different
units. Ion traps generally are important
in native MS workflows because of their ability to accumulate ions and perform
multi-stage analyses including fragmentation, photoreactions, and gas-phase
reactions with reagent molecules or ions.
Native MS, however, has shortcomings primarily due to the preferred
ionization technique, electrospray ionization (ESI). ESI tends to distribute signal from a single
analyte among a range of charge states.
Additionally, the ions generated from droplets tend to carry adducted
molecules and ions proportional to the size of the analyte. For analysis of high mass and heterogeneous
biomolecular complexes, these shortcomings lead to wide overlapping charge
states for different components that might be difficult to interpret correctly. Charge reduction via gas-phase ion/ion
reactions facilitates interpretation of native mass spectra by generating
product ions that are well separated in <i>m/z</i>. Current sine wave technology limits the upper
<i>m/z</i> range of ion traps required for stabilizing and measuring high mass
ion/ion reaction products. Digital ion
trapping (DIT) technology circumvents the voltage limitations of sine wave
technology by varying frequency to achieve high <i>m/z</i>. The combination of ion/ion reactions and DIT
operation facilitates further unique probing reactions such as fragmentation
reactions of charge reduced biomolecular complexes via neutral collisions and
photoreactions. DIT operation also
provides a straightforward approach for isolation of high <i>m/z</i> ions using
duty cycle modulation to further facilitate analysis of heterogeneous
mixtures. This work highlights
developments of a home-built 3D ion trap mass spectrometer as a viable native
MS platform.</p>
| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/13350857 |
| Date | 15 December 2020 |
| Creators | Kenneth W Lee (9746387) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | CC BY 4.0 |
| Relation | https://figshare.com/articles/thesis/DEVELOPMENT_OF_A_3D_ION_TRAP_FOR_ION_ION_REACTIONS_AND_MASS_ANALYSIS_INVOLVING_HIGH_MASS_BIOMOLECULAR_IONS/13350857 |
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