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Separation and Characterization of Variant Forms of Phosphoglucose Isomerase: Purification and Structural Analysis of Active Site Peptides from Human and Rabbit Phosphoglucose Isomerase

A method has been developed for the rapid, quantitative separation of normal and abnormal phosphoglucose isoemrase allozymes from individuals heterozygous for genetic variant forms of the enzyme. The method utilizes a substrate gradient elution of the enzyme from carboxymethyl Biogel and is far superior in terms of resolution and recovery to methods based on electrophoresis and isoelectric focusing. Four different genetic variant forms of the enzyme were isolated and subjected to a systematic comparison of their physical, catalytic and stability properties. The physical and catalytic properties of the variants were similar; however, clear differences in the stability of the allozymes were apparent.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc332219
Date05 1900
CreatorsGibson, David R.
ContributorsGracy, Robert W., Harris, Ben G., Jacobson, Myron, Glaze, William, Marshall, James L., 1940-
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatix, 195 leaves : ill., Text
RightsPublic, Gibson, David R., Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved.

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