Return to search

Hodnocení obsahu glutathionu v rostlinách jako markeru znečištění životního prostředí těžkými kovy / Evaluation of glutathione content in plants as a marker of heavy metals environmental contamination

Dependence of glutathione concentration on the amount of thallium in the plant was studied. Observed plant was maize (Zea mays) which was divided to two parts – root and overground. Two culture procedures were elaborated where seeds and young seedlings were cultivated in a solution of thallium of concentration 0, 1, 3, 5, 8, a 10 µmol/l. Extraction agents used during extraction were phosphate buffer and solution of ascorbic acid. Determination of glutathione was realized by capillary electrophoresis (CE) and high performance liquid chromatography (HPLC). Diode array detector (DAD) was used in both methods. Quantification of the thallium amount in the plant was done by method of inductively coupled plasma-atomic emission spectrometry (ICP-AES).

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:216355
Date January 2008
CreatorsBorková, Marie
ContributorsStoupalová, Michaela, Opatřilová,, Radka
PublisherVysoké učení technické v Brně. Fakulta chemická
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

Page generated in 0.0015 seconds