The synergistic effect between each of two minor contaminating UV wavelengths has been studied. The TC-7 host cell-capacity system was used. The macromolecular damage induced in monolayers of cell cultures as the result of exposure to UV radiation was measured by determining the ability of irradiated cells to support the replication of Herpes simplex virus. The macromolecule involved has been shown to be DNA and the damage is probably due to the formation of thymine dimers plus some undefined chromosphore associated with the DNA of the cell.
Preliminary experiments showed that low exposures of germicidal radiation are less effective in decreasing the survival of capacity while higher exposures are more effective when compared to monochromatic 254 nm radiation. Subsequent experiments showed a synergistic interaction between 254 nm and either 295 nm or 313 nm radiation at doses comparable to those emitted from a germicidal lamp as well as greatly increased doses.
| Identifer | oai:union.ndltd.org:WKU/oai:digitalcommons.wku.edu:theses-2898 |
| Date | 01 August 1978 |
| Creators | Cobb, Bobby |
| Publisher | TopSCHOLAR® |
| Source Sets | Western Kentucky University Theses |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Masters Theses & Specialist Projects |
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