The aim of this thesis is to observe the possibility of transfer of genetic information from feed, made of wheat with unusual grain colour and wheat with standard grain colour, into the blood of rat test subjects. Out of 64 rats that were tested, 32 were fed with purple grain (Konini variety) and formed the test group. A further 32 rats represented the study's control group and were fed with standard coloured wheat (Bohemia variety). The blood sample was acquired from the test animals. From 12 samples (6 samples from each group) DNA was isolated. Plant DNA was detected in the samples of DNA of rats by PCR using primers amplifying fragments of housekeeping genes specific for DNA of wheat (wheat genes Gapdh and Actin). No DNA fragments of wheat were detected in the blood of the rats. The results show, that there is no transfer of genetic information from the feed through the gastrointestinal tract to the blood of the animal.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:250508 |
| Date | January 2015 |
| Creators | Kovaříková, Helena |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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