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Sledování migrace mesenchymálních kmenových buněk v extracelulární matrix / Monitoring of mesenchymal stem cell migration in the extracellular matrix

This master’s thesis contains a description of mesenchymal stem cells (MSC). The work includes knowledge about the process of migration and differentiation of MSCs. The theoretical part of this thesis also deals with the problem of cell cultivation in 2D and 3D environments. The most used materials for creating 3D scaffolds are described here. The practical part contains the description of the cell culture protocol for passaging and also describes the experiment in the cell laboratory. The results of the experiment are discussed using confocal microscope images. The proposed experiment was tested with a sufficient number of repetitions. The processing of microscopic images was performed in the MATLAB programming environment.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:413027
Date January 2020
CreatorsZumberg, Inna
ContributorsČmiel, Vratislav, Chmelíková, Larisa
PublisherVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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