Diploma thesis deals with the problem of observation of controlled and spontaneous fermentation influence on the organic acids content in species of wine (Ruland blue) originated in subregion of Velké Pavlovice. Initial conditions of analysis (introduced in [41] and in materials concerning chromatographic column) were changed during lasting of experiment in such manner to get the most precise analysis of the wine sample. Enzyme Rapidase Excolor was added in the beginning of both types of fermentation plus yeasts were added at the controlled fermentation. Analysis of four organic acids was done; these were tartaric, malic, lactic and citric acid. High performance liquid chromatography (HPLC) with UV detection method was used to determine concentration of each acid. Wine was processed by two technological methods – by spontaneous and controlled fermentation. Each of the process differed in the organic acids content. Concentration of tartaric acid varied from 1,63 g/l to 6,43 g/l during spontaneous fermentation process. Decrease was caused by biological processes taking place. Concentration of citric acid was not almost influenced by the process of fermentation. Due to malolactic fermentation being in progress, decrease in malic acid concentration (from 6 g/l to 1,87 g/l) and increase in lactic acid concentration was observed. Concentration of tartaric acid varies as well during controlled fermentation. Biggest increase in concentration was observed at hour 214 of the experiment when the process of fermentation was supported by addition of lactic bacteria. Due to malolactic fermentation being in progress, decrease of malic acid concentration and increase of lactic acid concentration was observed similar to simultaneous fermentation. Concentration of citric acid was constant during controlled fermentation. Experiment proved that there is higher content of observed organic acids during controlled fermentation than during processes of spontaneous fermentation.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:216556 |
| Date | January 2009 |
| Creators | Křižánková, Hana |
| Contributors | Vespalcová, Milena, Hrstka, Miroslav |
| Publisher | Vysoké učení technické v Brně. Fakulta chemická |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
Page generated in 0.0013 seconds