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Previous issue date: 2016-03-31 / CAPES / The anticipation of the breeding season has been the subject of great interest in front of their
economic impact. In order to advance the first ovulation and anticipate the reproductive phase
of the mares, this study aimed to evaluate if the transvaginal follicle aspiration technique
guided by ultrasound during spring transition period, was able to induce cyclicity in mares.
The experiment was conducted in Animal Reproduction area of DRAA / IZ / UFRRJ, located
at Serop?dica-Rj, during spring transition period (August-September) and early reproductive
period (October) 2015. Selected mares were randomly distributed into three groups: Group 1
Control (G1, n = 9), without hormonal treatment; Group 2 P4 + PGF2? (G2, n = 9), 1500 mg
of long-acting progesterone, intramuscularly (IM), and seven days later, 7.5 mg Dinoprost
(PGF2?), IM and Group 3 transvaginal follicular aspiration + PGF2? (G3, n = 9), greater
follicle aspirated (>25mm) and seven days later, 7.5 mg Dinoprost (PGF2?), IM. Prior to the
beginning of the experiment, all mares were evaluated by ultrasonography, and the criteria
for the study was absence of corpora lutea as well as the presence of ovarian follicles larger
than 25 mm. Subsequent to this evaluation, the reproductive activity was monitored every 48
hours until the second ovulation of each mare. When evaluated the number of mares ovulating
within 16 days after initiation of treatment, it was observed that the groups G2 and G3 were
significantly more effective (P = 0.0031) in the first ovulation of the breeding season, when
compared to G1. In G1, 16 days after the start of treatment, no animal ovulated. In G2 and G3
eigh (88.9%) and six (66.7%) mares ovulated respectively. The mares were evaluated until the
second subsequent ovulation treatment. All mares ovulated normally, showing that none of
them returned to the transitional period and the average number of days between the first and
second ovulation did not differ between the groups (P> 0.05). The results of this study allow
us to conclude that follicular aspiration technique during spring transition period associated
with the administration of 7.5 mg of PGF2? seven days later, was able to induce cyclicity in
XI
mares. This treatment was effective as the conventional protocol P4 + PGF2?, thus possible to
use it in routine reproductive centers. However, more studies are needed to evaluate its
effectiveness during the early spring transition period, as well as its association with ovulation
inducers. / A antecipa??o da esta??o reprodutiva tem sido assunto de grande interesse frente ao seu
impacto econ?mico. A fim de adiantar a primeira ovula??o e antecipar a fase reprodutiva das
?guas, o presente estudo teve como objetivo avaliar se a t?cnica de aspira??o folicular
transvaginal guiada por ultrassom durante o per?odo de transi??o primaveril foi capaz de
induzir a ciclicidade em ?guas. O experimento foi realizado na ?rea de Reprodu??o Animal do
DRAA/IZ/UFRRJ, localizado no munic?pio de Serop?dica-RJ, durante o per?odo de transi??o
primaveril (agosto-setembro) e in?cio do per?odo reprodutivo (outubro) de 2015. Foram
selecionadas 27 ?guas da ra?a Mangalarga Marchador, entre 5-12 anos, pesando entre 350-
450 kg e com hist?rico de atividade reprodutiva normal. As ?guas selecionadas foram
distribu?das aleatoriamente em tr?s grupos: Grupo 1- Controle (G1; n=9), sem tratamento
hormonal; Grupo 2- P4 + PGF2? (G2; n=9), 1500mg de progesterona de longa a??o, por via
intramuscular (IM), e sete dias ap?s, 7,5 mg de Dinoprost (PGF2?), IM e Grupo 3- Aspira??o
folicular transvaginal + PGF2? (G3; n=9), maior fol?culo aspirado (>25mm) e sete dias ap?s,
7,5 mg de PGF2?, IM. Previamente ao in?cio do experimento, todas as ?guas foram avaliadas
por meio da ultrassonografia, sendo crit?rio para o estudo a aus?ncia de corpo l?teo, bem
como a presen?a de fol?culos ovarianos >25mm. Posteriormente a esta avalia??o, a atividade
reprodutiva foi monitorada a cada 48 horas at? o momento da segunda ovula??o de cada ?gua.
Quando avaliado o n?mero de ?guas que ovularam em at? 16 dias ap?s o in?cio do tratamento,
observou-se que os grupos G2 e G3 foram mais eficientes (P=0,0031) em acelerar a primeira
ovula??o da esta??o reprodutiva, quando comparados ao grupo G1. No grupo G1, ap?s 16
dias do in?cio do tratamento, nenhum animal ovulou. Nos grupos G2 e G3 oito (88,9%) e seis
(66,7%) ?guas ovularam, respectivamente. Analisando o n?mero m?dio de dias a partir do
tratamento at? a primeira ovula??o, os grupos tratados (G2 e G3) foram mais eficientes que o
IX
grupo controle na antecipa??o da primeira ovula??o (P<0,001). Em rela??o aos grupos
tratados, n?o houve diferen?a (P>0,05) entre eles. Foi poss?vel verificar que os grupos G2 e
G3 apresentaram uma antecipa??o da primeira ovula??o em rela??o ao controle em m?dia de
12,2 e 8,9 dias, respectivamente. As ?guas foram acompanhadas at? a segunda ovula??o
subsequente ao tratamento. Todas ovularam normalmente, demonstrando que nenhuma delas
retornou ao per?odo de transi??o e que a m?dia de dias entre a primeira e a segunda ovula??o,
n?o diferiu entre os grupos (P>0,05). Os resultados do presente estudo nos permitem concluir
que a t?cnica de aspira??o folicular durante o per?odo de transi??o primaveril, associado ?
administra??o de 7,5 mg de PGF2? sete dias mais tarde, foi capaz de induzir a ciclicidade em
?guas. Este tratamento mostrou-se t?o eficaz quanto o protocolo convencional de P4 + PGF2?,
sendo vi?vel utiliza-lo na rotina de centros reprodutivos. No entanto, mais estudos s?o
necess?rios a fim de avaliar sua efic?cia em fol?culos com di?metros menores no per?odo de
transi??o primaveril, assim como sua associa??o aos indutores de ovula??o.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1972 |
| Date | 31 March 2016 |
| Creators | DUTRA, Gabriel Almeida |
| Contributors | Jacob, Albuquerque, Flamarion Ten?rio de, Mello, Marco Roberto Bourg de |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Medicina Veterin?ria (Patologia e Ci?ncias Cl?nicas), UFRRJ, Brasil, Instituto de Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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