Monoclonal antibodies were developed against USDA
challenge strain of infectious laryngotracheitis virus (ILTV).
Indirect immunofluorescence test was used to detect antibodies
in supernatants of hybridomas. Hybridoma cells were developed
by fusing Sp 2/0 myeloma cells with spleen cells obtained from
mice immunized four times with partially purified USDA
challenge strain of infectious laryngotracheitis virus. The
supernatant of three hybridomas, designated as 2D1D8, 2E11G2,
2C6C7 were found positive for antibody activity against USDA
challenge strain of ILTV. Hybridomas producing antibodies were
cloned by the limiting dilution method.
All three monoclonal antibodies reacted with USDA
challenge strain of ILTV, S 88 00224 strain of ILTV, and 86
1169 strain of ILTV in an indirect immunofluorescence test.
None of the monoclonal antibodies reacted with avian
adenovirus 301 or parrot herpes virus in an indirect
immunofluorescence test. The monoclonal antibodies were
isotyped, and all three monoclonal antibodies were found to be
IgM. / Graduation date: 1993
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/35930 |
| Date | 28 October 1992 |
| Creators | Abbas, Ferhat, 1962- |
| Contributors | Andreasen, James R. Jr |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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