Two isolates (A and B) of apple chlorotic leafspot virus (ACLSV) associated with line pattern symptoms in Prunus, glandulosa were isolated in Chenopodium quinoa and purified by a simple method using bentonite clarification, polyethylene glycol precipitation, and caesium sulphate isopycnic centrifugation. Symptoms on herbaceous hosts were as previously reported for ACLSV. No symptoms were observed on a range of ACLSV woody host indicators.
Isolates A and B had a coat protein MW of 26 and 24.5 kilodaltons respectively, ssRNA of MW 2.6 x 10⁶ daltons, and dsRNA of MW 5.6 x 10⁶, 4.9 x 10⁶, and 4.5 x 10⁶ daltons. The particle widths were 12 nm and lengths were 782 nm (A) and 732 nm (B). Buoyant density in caesium sulphate was 1.27 g/cc³. In thin sections of C. quinoa, flexuous rods were seen in the cytoplasm and nucleus of young sieve-tube members. Polyclonal antisera prepared against the A and B isolates had high background reactions and required cross-adsorption with host sap. Three monoclonal antibodies (MAB) against isolate A detected Prunus strains while a fourth detected both Malus and Prunus strains in C. quinoa with low background reactions. The broad spectrum MAB could not be used as a trapping antibody but could be directly conjugated with alkaline phosphatase or used in an indirect triple-antibody sandwich ELISA.
Tobacco mosaic virus (TMV) recovered from P. glandulosa was identical in serological and physical properties to the TMV-U1 type strain. / Land and Food Systems, Faculty of / Graduate
| Identifer | oai:union.ndltd.org:UBC/oai:circle.library.ubc.ca:2429/29035 |
| Date | January 1990 |
| Creators | Thompson, Daniel Andrew |
| Publisher | University of British Columbia |
| Source Sets | University of British Columbia |
| Language | English |
| Detected Language | English |
| Type | Text, Thesis/Dissertation |
| Rights | For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use. |
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