La leptospirosis es una zoonosis bacteriana de gran impacto en la salud pública a nivel mundial. Los perros y otros animales, pueden infectarse, sufrir la enfermedad, constituyendo así, un factor importante en la diseminación de la bacteria hacia humanos. La infección es causada por cualquiera de los 250 serovares patógenos, de los 25 serogrupos de Leptospira spp. El estudio tuvo como objetivo identificar a los serogrupos de Leptospira spp. presentes en caninos domésticos. Para ello, se obtuvieron 305 muestras de suero sanguíneo, de perros con diagnóstico clínico presuntivo de Leptospirosis, mayores de 6 meses de edad, no vacunados o con un tiempo mayor a seis meses desde la última vacunación contra Leptospirosis. Las muestras fueron remitidas por Médicos Veterinarios al Laboratorio de Microbiología – Sección Bacteriología de la Facultad de Medicina Veterinaria de la Universidad Nacional Mayor de San Marcos, provenientes de 31 distritos de Lima Metropolitana. Se realizó la Prueba de Microaglutinación (MAT), estableciéndose títulos ≥1/100 de serorreactividad como seropositivos, utilizando cepas de referencia de los 25 serogrupos, incluyendo al nuevo serogrupo Iquitos serovar varillal 10, aislado y reportado en casos humanos en Perú. Se detectaron 177 seropositivos (58.03%), de los cuales 31 (10.16%) fueron coaglutinaciones. Los serogrupos de mayor frecuencia fueron: Iquitos (46/305; 15.08%), Tarassovi (37/305; 12.13%), Canicola (37/305; 12.13%), Australis (14/305; 4.59%), Icterohaemorrhagiae (13/305; 4.26%), Pomona (12/305; 3.93%), Mini (10/305; 3.28%) y Ballum (8/305; 2.62%). No se identificó serorreactores a los serugrupos Bataviae, Celledoni, Hebdomadis, Lousiana, Panama, Ranarum, Sarmin.
Palabras claves: Perros, leptospirosis, leptospira, MAT, serovar, serogrupo / Leptospirosis is an important bacterial zoonosis on worldwide. Dogs and other animals can be infected and suffered the disease, they constituting an important role in the spread of the bacteria to humans. The infection is caused by any of the 250 pathogenic serovars, grouped in 25 serogroups of Leptospira spp. The aim of this study was to identify the serogroups of Leptospira spp. circulating in domestic dogs. For this, 305 serum samples were obtained from dogs, who had a presumptive clinical diagnosis of Leptospirosis, over 6 months old, unvaccinated or more than six months since the last vaccination against Leptospirosis, The samples were submitted by veterinarians to Microbiology Lab - Bacteriology, Faculty of Veterinary Medicine of Universidad Nacional Mayor de San Marcos, from 31 districts of Lima. The microagglutination test (MAT) was performed, establishing seroreactivity titles ≥ 1/100 as seropositive using reference strains of the 25 serogroups, including the new serogroup Iquitos, serovar Varillal, Var 10 strain, isolated and reported human outbreaks in Peru. 177 seropositive samples (58.03%) were detected, 31 of them (10.16%) were co-agglutinations. Seropositive reacted against 18 serogroups, the most frequent were: Iquitos (46/305; 15.08%), Tarassovi (37/305; 12.13%), Canicola (37/305; 12.13%), Australis (14/305; 4.59%), Icterohaemorrhagiae (13/305; 4.26%), Pomona (12/305; 3.93%), Mini (10/305; 3.28%) and Ballum (8/305; 2.62%). There were no seroreactors to serogroups: Bataviae, Celledoni, Hebdomadis, Lousiana, Panama, Ranarum, and Sarmin.
Keywords: Dog, leptospirosis, leptospira, MAT, serovar, serogroup.
Identifer | oai:union.ndltd.org:Cybertesis/oai:cybertesis.unmsm.edu.pe:cybertesis/3999 |
Date | January 2014 |
Creators | Siuce Moreno, Juan José |
Publisher | Universidad Nacional Mayor de San Marcos |
Source Sets | Universidad Nacional Mayor de San Marcos - SISBIB PERU |
Language | Spanish |
Detected Language | Spanish |
Type | info:eu-repo/semantics/masterThesis |
Source | Repositorio de Tesis - UNMSM, Universidad Nacional Mayor de San Marcos |
Rights | info:eu-repo/semantics/openAccess |
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