This thesis is focused on verifying in vitro cultivation techniques in micropropagation of tropical terrestrial orchid Macodes petola (Bl.) Lindl. The aim of the studies was to verify the responsiveness of the node explants of Macodes petola (Bl.) Lindl to different surface-sterilization methods and duration of sterilization time, as well as the transport of the node explants to agar medium. Node explants of Macodes petola (Bl.) Lindl were surface-sterilized with different disinfectants for different sterilization time and cultivated in primordial culture. The best results were observed by using sequential surface sterilization in ethyl alcohol (70%) and disinfectant Savo (2.5%) - ethyl alcohol being used for 15 seconds and Savo for 20 minutes.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:48058 |
| Date | January 2011 |
| Creators | ATTLOVÁ, Irena |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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