In the search for new genes that are regulated by thyroid hormone, DISP3, a new member of the SSD (sterol-sensing domain) protein family, has been identified. Expression analysis showed that DISP3 is expressed in cells of neural origin, and our previous results indicate that overexpression of this gene affects cell proliferation and differentiation. Oncomine database analysis also showed that DISP3 expression is increased in medulloblastomas, the most common malignancies of the central nervous system in children. The subject of this diploma thesis is studying the effect of increased DISP3 levels on cell apoptosis and cell ability to form a colony. Cell lines derived from medulloblastomas were used in the work. We compared the expression levels of the DISP3 gene in different medulloblastoma lines by quantitative PCR and selected a line with low expression of this gene for further experiments. Some medulloblastoma cell lines can form neurospheres when cultured in serum-free medium. Using quantitative PCR, we compared the expression levels of neural markers in cells cultured both as neurospheres and as adherent cells. By transfecting cells with a plasmid overexpressing DISP3, we prepared cells with increased levels of this gene. We induced apoptosis by radiation at different doses. Apoptosis was...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:435864 |
| Date | January 2020 |
| Creators | Jarošová, Šárka |
| Contributors | Zíková, Martina, Havránek, Ondřej |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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