This study had a twofold purpose. First, the usefulness of the simplex algorithm as a short method of optimization in high performance liquid chromatographic separations was investigated. The second was to test a modified simplex method. The volume fractions of mobile phase components were chosen as the variable factors in the optimization process. Four test cases were performed which included separation of cholesterol esters, naphthalene and its derivatives, polycyclic aromatic compounds, and the thiane compounds. The standard for accepting an optimum was based on the baseline separation of two adjacent peaks and the analysis time. In addition to successful separations, the correlation between the separation and the chemical characteristics of mobile phase compositions was calculated and this could then be used for further modification of simplex search strategy.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc504387 |
| Date | 08 1900 |
| Creators | Nguyen, Khanh Thi |
| Contributors | Thompson, Richard E., Jones, Paul R. |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | ix, 111 leaves: ill., Text |
| Rights | Public, Nguyen, Khanh Thi, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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