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Study of zooplankton feeding selectivity by HPLC analysis of phytoplankton pigment.

Siu Yuen Yu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 122-139). / Abstracts in English and Chinese. / Abstract (English) --- p.i / Abstract (Chinese) --- p.iii / Acknowledgments --- p.v / Table of Contents --- p.vi / List of Figures --- p.x / List of Tables --- p.xvi / Chapter CHAPTER 1 --- INTRODUCTION --- p.1 / Chapter CHAPTER 2 --- LITERATURE REVIEW --- p.7 / Chapter 2.1 --- Traditional methods for studying zooplankton feeding selectivity --- p.7 / Chapter 2.1.1 --- Cell counting after laboratory feeding experiments --- p.7 / Chapter 2.1.2 --- Direct examination of gut contents --- p.8 / Chapter 2.1.3 --- Use of radioactive tracers --- p.9 / Chapter 2.1.4 --- Gut fluorescence method --- p.9 / Chapter 2.2 --- High Performance Liquid Chromatography analysis of phytoplankton pigments --- p.11 / Chapter 2.2.1 --- Principle --- p.11 / Chapter 2.2.2 --- Pigments as signature markers of phytoplankton --- p.11 / Chapter 2.2.3 --- Development of HPLC analysis of phytoplankton pigments --- p.16 / Chapter 2.2.4 --- Advantages of HPLC analysis of phytoplankton pigments --- p.17 / Chapter 2.2.5 --- Limitation of HPLC analysis of phytoplankton pigments --- p.18 / Chapter 2.3 --- Zooplankton feeding selectivity --- p.19 / Chapter 2.3.1 --- Ecological importance of zooplankton feeding selectivity --- p.19 / Chapter 2.3.2 --- Factors affecting zooplankton feeding selectivity --- p.19 / Chapter 2.3.3 --- Feeding selectivity of zooplankton studied in this study --- p.21 / Chapter 2.3.3.1 --- p. avirostirs --- p.21 / Chapter 2.3.3.2 --- Paracalanus spp --- p.22 / Chapter 2.3.3.3 --- Pseudevadne tergestina --- p.23 / Chapter 2.4 --- Pigment degradation in guts of zooplankton --- p.24 / Chapter 2.4.1 --- Experimental design --- p.24 / Chapter 2.4.2 --- Pigment degradation --- p.24 / Chapter 2.5 --- "Tolo Harbour, Hong Kong" --- p.26 / Chapter 2.5.1 --- Site description --- p.26 / Chapter 2.5.2 --- Phytoplankton and zooplankton in Tolo Harbour --- p.27 / Chapter CHAPTER 3 --- MATERIALS AND METHODS --- p.28 / Chapter 3.1 --- Field sampling --- p.28 / Chapter 3.1.1 --- Study of seasonal patterns in zooplankton feeding selectivity --- p.28 / Chapter 3.1.1.1 --- Collection of phytoplankton and zooplankton for pigment analysis --- p.28 / Chapter 3.1.1.2 --- Collection of phytoplankton and zooplankton for plankton enumeration --- p.30 / Chapter 3.1.2 --- Collection of phytoplankton and zooplankton for laboratory feeding experiments --- p.32 / Chapter 3.2 --- Laboratory experiments and data analysis --- p.33 / Chapter 3.2.1 --- Study of seasonal patterns in zooplankton feeding selectivity --- p.33 / Chapter 3.2.1.1 --- HPLC of phytoplankton pigments --- p.33 / Chapter 3.2.1.2 --- Fluorometric measurement of chlorophyll-α --- p.35 / Chapter 3.2.1.3 --- Plankton identification and enumeration --- p.36 / Chapter 3.2.2 --- Laboratory feeding experiments for investigation of pigment degradation in zooplankton gut --- p.37 / Chapter CHAPTER 4 --- RESULTS --- p.41 / Chapter 4.1 --- Information on Tolo Harbour --- p.41 / Chapter 4.1.1 --- Temperature and salinity in Tolo Harbour --- p.41 / Chapter 4.1.2 --- Plankton composition and community in Tolo Harbour --- p.43 / Chapter 4.1.2.1 --- Phytoplankton --- p.43 / Chapter 4.1.2.2 --- Zooplankton --- p.50 / Chapter 4.2 --- Seasonal zooplankton feeding selectivity investigated by HPLC phytoplankton pigment analysis --- p.53 / Chapter 4.2.1 --- Verification of HPLC pigment analysis by fluorometric analysis --- p.53 / Chapter 4.2.2 --- Correlations between phytoplankton cell densities and pigment concentrations in water samples --- p.55 / Chapter 4.2.3 --- Feeding selectivity of zooplankton on different phytoplankton groups --- p.73 / Chapter 4.2.4 --- Feeding selectivity of zooplankton on dinoflagellates --- p.87 / Chapter 4.2.5 --- Feeding selectivity of zooplankton on diatoms --- p.87 / Chapter 4.3 --- Feeding selectivity on phytoplankton by other cladoceran - Pseudevadne tergestina --- p.89 / Chapter 4.4 --- Pigment degradation in zooplankton guts after ingestion of phytoplankton --- p.90 / Chapter 4.5 --- Clearance rates of P. avirostris and Paracalanus spp. in feeding experiments --- p.101 / Chapter CHAPTER 5 --- DISCUSSIONS --- p.105 / Chapter 5.1 --- Experiment design --- p.105 / Chapter 5.2 --- Seasonal zooplankton feeding selectivity investigated by HPLC phytoplankton pigment analysis --- p.108 / Chapter 5.2.1 --- Correlations between phytoplankton cell densities and pigment concentrations in water samples --- p.108 / Chapter 5.2.2 --- Feeding selectivity of zooplankton on different phytoplankton groups --- p.108 / Chapter 5.2.3 --- Feeding selectivity of Pseudevadne tergestina --- p.111 / Chapter 5.3 --- Feeding experiments for investigating pigment degradation in guts of zooplankton --- p.112 / Chapter 5.3.1 --- Principle --- p.112 / Chapter 5.3.2 --- Degradation for different pigments in guts of P. avirostris and Paracalanus spp. --- p.112 / Chapter 5.4 --- Clearance rates of P. avirostris and Paracalanus spp. --- p.114 / Chapter 5.4.1 --- p. avirostris --- p.114 / Chapter 5.4.2 --- Paracalanus spp. --- p.115 / Chapter 5.5 --- Limitations of HPLC analysis of phytoplankton pigments --- p.116 / Chapter 5.6 --- Environmental events related to feeding selectivity of zooplankton in Tolo Harbour --- p.118 / Chapter 5.6.1 --- Energy transfer in trophic level --- p.118 / Chapter 5.6.2 --- Abilities of p. avirostris and Paracalanus spp. to control red tides in Tolo Harbour --- p.118 / Chapter CHAPTER 6 --- CONCLUSION --- p.120 / REFERENCES --- p.122 / APPENDIX

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324656
Date January 2004
ContributorsSiu, Yuen Yu., Chinese University of Hong Kong Graduate School. Division of Biology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xvi, 140 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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