Varying concentrations of the food preservatives nisin and lysozyme were
adsorbed onto glass surfaces chemically modified to exhibit different degrees of
hydrophobicity. The antimicrobial activity of the adsorbed preservatives was evaluated
by documenting the ability of Listeria monocytogenes to adhere and grow on the glass
surfaces. A bioluminescence protocol was developed to effectively enumerate bacterial
cells adhered to glass. Lysozyme adsorption onto glass surfaces was monitored by
labeling with ¹²⁵I.
Results indicated that synergy was present for 0.9/0.1 molecular ratio of
nisin/lysozyme. Synergistic effect was increasing gradually with the increase of nisin in
the ratios tested. This trend was observed on both surface types. However, the magnitude
of synergy was more pronounced on hydrophobic surfaces than on hydrophilic ones.
Results from protein radiolabeling showed that lysozyme was adsorbed with higher mass
to hydrophilic surfaces than to hydrophobic ones. / Graduation date: 1999
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/27253 |
| Date | 16 November 1998 |
| Creators | Jaczynski, Jacek |
| Contributors | Daeschel, Mark |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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