Lo Kam Chun. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 310-323). / Abstracts in English and Chinese. / Abstract --- p.i / Abstract (Chinese version) --- p.iii / Acknowledgements --- p.v / Table of Contents --- p.vi / List of Abbreviations --- p.xxvi / List of Unit Abbreviations --- p.xxviii / List of Figures --- p.xxix / List of Tables --- p.xxxviii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter Chapter 2 --- Literature Review --- p.3 / Chapter 2.1 --- Peroxisome proliferator-activated receptors (PPARs) --- p.3 / Chapter 2.1.1 --- Structure of PPAR --- p.4 / Chapter 2.1.2 --- Ligands of PPAR --- p.6 / Chapter 2.1.3 --- Peroxisome proliferator responsive elements (PPREs) --- p.8 / Chapter 2.1.4 --- Coactivators and corepressors of PPAR --- p.8 / Chapter 2.1.5 --- Signaling cross-talk between PPAR and other nuclear hormone receptors --- p.11 / Chapter 2.1.6 --- Tissue distribution of PPAR --- p.12 / Chapter 2.2 --- PPARα and lipid metabolism --- p.13 / Chapter 2.3 --- "PPARα, cholesterol and lipoprotein metabolism" --- p.18 / Chapter 2.4 --- PPARα and glucose metabolism --- p.20 / Chapter 2.5 --- PPARα and amino acid metabolism --- p.21 / Chapter 2.6 --- "PPARα, atherosclerosis and inflammation" --- p.21 / Chapter 2.7 --- PPARα and epidermal differentiation --- p.23 / Chapter 2.8 --- PPARα and peroxisome proliferator-induced liver injury --- p.24 / Chapter Chapter 3 --- Objectives --- p.25 / Chapter Chapter 4 --- Materials and Methods --- p.26 / Chapter 4.1 --- Animal tail-genotyping --- p.26 / Chapter 4.1.1 --- Materials --- p.26 / Chapter 4.1.2 --- Methods --- p.26 / Chapter 4.2 --- Animal treatment --- p.29 / Chapter 4.2.1 --- Animals --- p.30 / Chapter 4.2.2 --- Treatment --- p.30 / Chapter 4.3 --- "Serum β-hydroxybutyrate, triglyceride and cholesterol analyses" --- p.30 / Chapter 4.3.1 --- Materials --- p.32 / Chapter 4.3.2 --- Methods --- p.32 / Chapter 4.3.2.1 --- Serum preparation --- p.32 / Chapter 4.3.2.2 --- β-hydroxybutyrate determination --- p.32 / Chapter 4.3.2.3 --- Triglyceride determination --- p.33 / Chapter 4.3.2.4 --- Cholesterol determination --- p.33 / Chapter 4.4 --- Total RNA isolation --- p.34 / Chapter 4.4.1 --- Materials --- p.34 / Chapter 4.4.2 --- Methods --- p.34 / Chapter 4.5 --- DNase I treatment --- p.35 / Chapter 4.5.1 --- Materials --- p.35 / Chapter 4.5.2 --- Methods --- p.35 / Chapter 4.6 --- Reverse transcription (RT) and non-fluorescent PCR (non-fluoroDD PCR) --- p.36 / Chapter 4.6.1 --- Materials --- p.41 / Chapter 4.6.2 --- Methods --- p.41 / Chapter 4.7 --- Reverse transcription (RT) and fluorescent PCR (fluoroDD PCR) --- p.42 / Chapter 4.7.1 --- Materials --- p.42 / Chapter 4.7.2 --- Methods --- p.42 / Chapter 4.8 --- Fluorescent differential display --- p.42 / Chapter 4.8.1 --- Materials --- p.43 / Chapter 4.8.2 --- Methods --- p.43 / Chapter 4.9 --- Excision of differentially expressed cDNA fragments --- p.44 / Chapter 4.9.1 --- Materials --- p.44 / Chapter 4.9.2 --- Methods --- p.44 / Chapter 4.10 --- Reamplification of excised cDNA fragments --- p.44 / Chapter 4.10.1 --- Materials --- p.45 / Chapter 4.10.2 --- Methods --- p.45 / Chapter 4.11 --- Subcloning of reamplified cDNA fragments --- p.46 / Chapter 4.11.1 --- Materials --- p.46 / Chapter 4.11.2 --- Methods --- p.46 / Chapter 4.12 --- Reverse dot blot analysis of subcloned cDNA fragments --- p.50 / Chapter 4.12.1 --- Materials --- p.50 / Chapter 4.12.2 --- Methods --- p.51 / Chapter 4.12.2.1 --- Mini-preparation of plasmid DNA from recombinant clones ´ؤ´ؤalkaline lysis method --- p.51 / Chapter 4.12.2.2 --- Dot blot preparation --- p.52 / Chapter 4.12.2.3 --- cDNA library and probe preparation --- p.52 / Chapter 4.12.2.4 --- Hybridization and signal detection --- p.54 / Chapter 4.13 --- Sequencing of reverse dot blot-confirmed cDNA fragments --- p.55 / Chapter 4.13.1 --- Materials --- p.55 / Chapter 4.13.2 --- Methods --- p.55 / Chapter 4.13.2.1 --- Mini-preparation of plasmid DNA from recombinant clones ´ؤ´ؤQiagen method --- p.55 / Chapter 4.13.2.2 --- DNA sequencing by CEQ dye terminator cycle sequencing --- p.56 / Chapter 4.13.2.3 --- BLAST search against computer database --- p.57 / Chapter 4.14 --- Northern blot analysis of sequenced cDNA fragments --- p.57 / Chapter 4.14.1 --- Materials --- p.58 / Chapter 4.14.2 --- Methods --- p.58 / Chapter 4.14.2.1 --- Midi-preparation of plasmid DNA from recombinant clones for probe preparation ´ؤ´ؤQiagen method --- p.58 / Chapter 4.14.2.2 --- DIG-labeled cDNA probe preparation --- p.59 / Chapter 4.14.2.3 --- Formaldehyde-agarose gel electrophoresis --- p.60 / Chapter 4.14.2.4 --- Hybridization and signal detection --- p.61 / Chapter Chapter 5 --- Results --- p.63 / Chapter 5.1 --- Confirmation of genotypes by PCR tail-genotyping --- p.63 / Chapter 5.2 --- Morphological observation --- p.63 / Chapter 5.3 --- "Serum β-hydroxybutyrate, triglyceride and cholesterol levels" --- p.67 / Chapter 5.4 --- RT and non-fluoroDD PCR --- p.69 / Chapter 5.5 --- RT and fluoroDD PCR --- p.94 / Chapter 5.6 --- Reamplification of cDNA fragments --- p.121 / Chapter 5.7 --- Subcloning of reamplified cDNA fragments --- p.131 / Chapter 5.8 --- Reverse dot blot analysis of subcloned cDNA fragments --- p.174 / Chapter 5.9 --- Sequencing of reverse dot blot-confirmed cDNA fragments --- p.243 / Chapter 5.10 --- Northern blot analysis of sequenced cDNA fragments --- p.262 / Chapter Chapter 6 --- Discussions --- p.282 / Chapter 6.1 --- PPARα and hepatic lipid metabolism during fasting --- p.282 / Chapter 6.2 --- Enlargement of liver in starved PPARα-null mice --- p.283 / Chapter 6.3 --- "Alteration of serum β-hydroxybutyrate, triglyceride and cholesterol levels by PPARa during fasting" --- p.284 / Chapter 6.4 --- Identification of PPARα-dependent and fasting- responsive genes by fluorescent differential display and PPARα-null mice --- p.285 / Chapter 6.4.1 --- Roles of PPARα-dependent and fasting-responsive genes --- p.289 / Chapter 6.4.1.1 --- Lipid metabolism --- p.290 / Chapter 6.4.1.2 --- Protein metabolism --- p.299 / Chapter 6.4.1.3 --- Other functions --- p.302 / Chapter Chapter 7 --- Conclusions --- p.307 / Chapter Chapter 8 --- Future Studies --- p.309 / Chapter 8.1 --- Further identification of fasting-responsive and PPARα- dependent genes --- p.309 / Chapter 8.2 --- Characterization of the mouse EST --- p.309 / References --- p.310 / Chapter Appendix A: --- Protocol for reaction preparation / Chapter Table A1 --- PCR-tail genotyping --- p.324 / Chapter Table A2 --- DNase I treatment of total RNA --- p.324 / Chapter Table A3 --- Reverse transcription (RT) for both non-fluoroDD and fluooDD PCR --- p.324 / Chapter Table A4 --- Non-fluoroDD PCR --- p.325 / Chapter Table A5 --- FluoroDD PCR --- p.325 / Chapter Table A6 --- Reamplification of cDNA fragments --- p.325 / Chapter Table A7 --- Reverse transcription in cDNA library synthesis for reverse dot blot analysis --- p.326 / Chapter Table A8 --- RT-PCR in cDNA library synthesis for reverse dot blot analysis --- p.326 / Chapter Appendix B: --- Phenol-chloroform extraction for recombinant subclones / Chapter Figure B1 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel D --- p.327 / Chapter Figure B2 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel E --- p.328 / Chapter Figure B3 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gels G and HH --- p.329 / Chapter Figure B4 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel JJ --- p.330 / Chapter Figure B5 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel LL --- p.331 / Chapter Figure B6 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel MM --- p.332 / Chapter Figure B7 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gels NN and OO (fragments OO1 - OO4) --- p.333 / Chapter Figure B8 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel 00 (fragments OO5 - OO7) --- p.334 / Chapter Figure B9 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel OO (fragments OO8 and OO9) --- p.335 / Chapter Figure B10 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel PP --- p.336 / Chapter Figure B11 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel QQ (fragments QQ1 and QQ3) --- p.337 / Chapter Figure B12 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel QQ (fragments QQ4 - QQ6) --- p.338 / Chapter Figure B13 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel RR --- p.339 / Chapter Figure B14 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gels SS and TT --- p.340 / Chapter Figure B15 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel UU --- p.341 / Chapter Figure B16 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel VV --- p.342 / Chapter Figure B17 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel WW --- p.343 / Chapter Figure B18 --- Phenol-chloroform extraction for recombinant subclones containing cDNA fragments excised from fluoroDD gel XX --- p.344 / Chapter Appendix C: --- DNA sequence and sequence alignment of cDNA subclones / Chapter C1.1 --- DNA sequence of cDNA subclone Dl#2 using Ml3 forward -20 primer and the sequence of 3´ة-AP3 --- p.345 / Chapter C1.2 --- Sequence alignment of cDNA subclone Dl#2 with mouse CYP4A10 by BLAST searching with the National Center for Biotechnology Information database --- p.345 / Chapter C1.3 --- Summary of sequence alignment of cDNA subclone Dl#2 with mouse CYP4A10 --- p.345 / Chapter C2.1 --- DNA sequence of cDNA subclone Dl#2 using Ml3 reverse primer and the sequence of 5´ة-ARP12 --- p.346 / Chapter C2.2 --- Sequence alignment of cDNA subclone Dl#2 with mouse CYP4A10 by BLAST searching with the National Center for Biotechnology Information database --- p.346 / Chapter C2.3 --- Summary of sequence alignment of cDNA subclone Dl#2 with mouse CYP4A10 --- p.346 / Chapter C3.1 --- DNA sequence of cDNA subclone D2#9 using Ml3 forward -20 primer and the sequence of 5'-ARP12 --- p.347 / Chapter C3.2 --- Sequence alignment of cDNA subclone D2#9 with mouse Bhmt by BLAST searching with the National Center for Biotechnology Information database --- p.347 / Chapter C3.3 --- Summary of sequence alignment of cDNA subclone D2#9 with mouse Bhmt --- p.347 / Chapter C4.1 --- DNA sequence of cDNA subclone D3#3 using Ml3 forward -20 primer and the sequence of 5'-ARP12 --- p.348 / Chapter C4.2 --- Sequence alignment of cDNA subclone D3#3 with mouse hypothetical protein MMT-7 by BLAST searching with the National Center for Biotechnology Information database --- p.348 / Chapter C4.3 --- Summary of sequence alignment of cDNA subclone D3#3 with mouse hypothetical protein MMT-7 --- p.348 / Chapter C5.1 --- DNA sequence of cDNA subclone D3#3 using M13 reverse primer and the sequence of 3´ة-AP3 --- p.349 / Chapter C5.2 --- Sequence alignment of cDNA subclone D3#3 with mouse hypothetical protein MMT-7 by BLAST searching with the National Center for Biotechnology Information database --- p.349 / Chapter C5.3 --- Summary of sequence alignment of cDNA subclone D3#3 with mouse hypothetical protein MMT-7 --- p.349 / Chapter C6.1 --- DNA sequence of cDNA subclone LL1#7 using Ml3 reverse primer and the sequence of 3´ة-AP4 --- p.350 / Chapter C6.2 --- Sequence alignment of cDNA subclone LL1#7 with mouse Ctsl by BLAST searching with the National Center for Biotechnology Information database --- p.350 / Chapter C6.3 --- Summary of sequence alignment of cDNA subclone LL1#7 with mouse Ctsl --- p.350 / Chapter C7.1 --- DNA sequence of cDNA subclone LL1#11 using Ml3 forward -20 primer and the sequence of 5'-ARP6 --- p.351 / Chapter C7.2 --- Sequence alignment of cDNA subclone LL1#11 with mouse Ctsl by BLAST searching with the National Center for Biotechnology Information database --- p.351 / Chapter C7.3 --- Summary of sequence alignment of cDNA subclone LL1#11 with mouse Ctsl --- p.351 / Chapter C8.1 --- DNA sequence of cDNA subclone MM1#1 using M13 forward -20primer and the sequence of 3'-AP2 --- p.352 / Chapter C8.2 --- Sequence alignment of cDNA subclone MM1#1 with mouse CYP4A10 by BLAST searching with the National Center for Biotechnology Information database --- p.352 / Chapter C8.3 --- Summary of sequence alignment of cDNA subclone MM1#1 with mouse CYP4A10 --- p.352 / Chapter C9.1 --- DNA sequence of cDNA subclone MM1#1 using Ml3 reverse primer and the sequence of 5´ة-ARP7 --- p.353 / Chapter C9.2 --- Sequence alignment of cDNA subclone MM1#1 with mouse CYP4A110 by BLAST searching with the National Center for Biotechnology Information database --- p.353 / Chapter C9.3 --- Summary of sequence alignment of cDNA subclone MM1#1 with mouse CYP4A10 --- p.353 / Chapter C10.1 --- DNA sequence of cDNA subclone 002#6 using Ml3 forward -20 primer and the sequence of 3'-AP9 --- p.354 / Chapter C10.2 --- Sequence alignment of cDNA subclone 002#6 with rat ASL by BLAST searching with the National Center for Biotechnology Information database --- p.354 / Chapter C10.3 --- Summary of sequence alignment of cDNA subclone 002#6 with rat ASL --- p.354 / Chapter C11.l --- DNA sequence of cDNA subclone 002#9 using Ml3 reverse primer and the sequence of 5'-ARP3 --- p.355 / Chapter C11.2 --- Sequence alignment of cDNA subclone 002#9 with rat ASL by BLAST searching with the National Center for Biotechnology Information database --- p.355 / Chapter C11.3 --- Summary of sequence alignment of cDNA subclone 002#9 with rat ASL --- p.355 / Chapter C12.1 --- DNA sequence of cDNA subclone 003#4 using Ml3 forward -20 primer and the sequence of 5'-ARP3 --- p.356 / Chapter C12.2 --- Sequence alignment of cDNA subclone O03#4 with mouse Phyh by BLAST searching with the National Center for Biotechnology Information database --- p.356 / Chapter C12.3 --- Summary of sequence alignment of cDNA subclone 003#4 with mouse Phyh --- p.356 / Chapter C13.1 --- DNA sequence of cDNA subclone 003#9 using Ml3 reverse primer and the sequence of 5'-ARP3 --- p.357 / Chapter C13.2 --- Sequence alignment of cDNA subclone 003#9 with rat ASL by BLAST searching with the National Center for Biotechnology Information database --- p.357 / Chapter C13.3 --- Summary of sequence alignment of cDNA subclone 003#9 with rat ASL --- p.357 / Chapter C14. --- DNA sequence of cDNA subclone 004#3 using M13 forward -20 primer and the sequence of 5'-ARP3 --- p.358 / Chapter C14.2 --- Sequence alignment of cDNA subclone 004#3 with rat ASL by BLAST searching with the National Center for Biotechnology Information database --- p.358 / Chapter C14.3 --- Summary of sequence alignment of cDNA subclone 004#3 with rat ASL --- p.358 / Chapter C15.1 --- DNA sequence of cDNA subclone 004#3 using Ml3 reverse primer and the sequence of 3'-AP9 --- p.359 / Chapter C15.2 --- Sequence alignment of cDNA subclone 004#3 with rat ASL by BLAST searching with the National Center for Biotechnology Information database --- p.359 / Chapter C15.3 --- Summary of sequence alignment of cDNA subclone 004#3 with rat ASL --- p.359 / Chapter C16.1 --- DNA sequence of cDNA subclone 004#4 using Ml 3 forward -20primer and the sequence of 5?-ARP3 --- p.360 / Chapter C16.2 --- Sequence alignment of cDNA subclone 004#4 with mouse CYP4A14 by BLAST searching with the National Center for Biotechnology Information database --- p.360 / Chapter C16.3 --- Summary of sequence alignment of cDNA subclone 004#4 with mouse CYP4A14 --- p.360 / Chapter C17.1 --- DNA sequence of cDNA subclone 004#4 using M13 reverse primer and the sequence of 3´ة-AP9 --- p.361 / Chapter C17.2 --- Sequence alignment of cDNA subclone 004#4 with mouse CYP4A14 by BLAST searching with the National Center for Biotechnology Information database --- p.361 / Chapter C17.3 --- Summary of sequence alignment of cDNA subclone 004#4 with mouse CYP4A14 --- p.361 / Chapter C18.1 --- DNA sequence of cDNA subclone 004#10 using M13 forward -20primer and the sequence of 3´ة-AP9 --- p.362 / Chapter C18.2 --- a Sequence alignment of cDNA subclone 004#10 with rat PBE by BLAST searching with the National Center for Biotechnology Information database --- p.362 / Chapter C18.3 --- a Summary of sequence alignment of cDNA subclone 004#10 with rat PBE --- p.362 / Chapter C18.2 --- b Sequence alignment of cDNA subclone 004#10 with mouse L-PBE by BLAST searching with the National Center for Biotechnology Information database --- p.363 / Chapter C18.3 --- b Summary of sequence alignment of cDNA subclone 004#10 with mouse L-PBE --- p.363 / Chapter C19.1 --- DNA sequence of cDNA subclone 005#13 using M13 forward -20primer and the sequence of 3'-AP9 --- p.364 / Chapter C19.2 --- Sequence alignment of cDNA subclone 005#13 with mouse CYP4A14 by BLAST searching with the National Center for Biotechnology Information database --- p.364 / Chapter C19.3 --- Summary of sequence alignment of cDNA subclone 005#13 with mouse CYP4A14 --- p.364 / Chapter C20.1 --- DNA sequence of cDNA subclone 005#13 using Ml3 reverse primer and the sequence of 5'-ARP3 --- p.365 / Chapter C20.2 --- Sequence alignment of cDNA subclone 005#13 with mouse CYP4A14 by BLAST searching with the National Center for Biotechnology Information database --- p.365 / Chapter C20.3 --- Summary of sequence alignment of cDNA subclone 005#13 with mouse CYP4A14 --- p.365 / Chapter C21.1 --- DNA sequence of cDNA subclone 006#2 using Ml3 forward -20 primer and the sequence of 3´ة-AP9 --- p.366 / Chapter C21.2 --- Sequence alignment of cDNA subclone 006#2 with mouse CYP4A14 by BLAST searching with the National Center for Biotechnology Information database --- p.366 / Chapter C21.3 --- Summary of sequence alignment of cDNA subclone 006#2 with mouse CYP4A14 --- p.366 / Chapter C22.1 --- DNA sequence of cDNA subclone 006#8 using Ml3 forward -20 primer and the sequence of 5'-ARP3 --- p.367 / Chapter C22.2 --- Sequence alignment of cDNA subclone 006#8 with mouse CYP4A14 by BLAST searching with the National Center for Biotechnology Information database --- p.367 / Chapter C22.3 --- Summary of sequence alignment of cDNA subclone 006#8 with mouse CYP4A14 --- p.367 / Chapter C23.1 --- DNA sequence of cDNA subclone 006#10 using Ml3 reverse primer and the sequence of 5'-ARP3 --- p.368 / Chapter C23.2 --- Sequence alignment of cDNA subclone 006#10 with mouse CYP4A14 by BLAST searching with the National --- p.368 / Chapter C23.3 --- Summary of sequence alignment of cDNA subclone 006#10 with mouse CYP4A14 --- p.368 / Chapter C24.1 --- DNA sequence of cDNA subclone 007#6 using Ml3 reverse primer and the sequence of 5'-ARP3 --- p.368 / Chapter C24.2 --- Sequence alignment of cDNA subclone 007#6 with mouse Spil-3 by BLAST searching with the National Center for Biotechnology Information database --- p.369 / Chapter C24.3 --- Summary of sequence alignment of cDNA subclone 007#6 with mouse Spil-3 --- p.369 / Chapter C25.1 --- DNA sequence of cDNA subclone 007#10 using Ml3 forward -20 primer and the sequence of 3'-AP9 --- p.370 / Chapter C25.2 --- Sequence alignment of cDNA subclone 007#10 with mouse Spil-3 by BLAST searching with the National Center for Biotechnology Information database --- p.370 / Chapter C25.3 --- Summary of sequence alignment of cDNA subclone 007#10 with mouse Spil-3 --- p.370 / Chapter C26.1 --- DNA sequence of cDNA subclone 008#10 using Ml3 forward -20 primer and the sequence of 5'-ARP3 --- p.371 / Chapter C26.2 --- Sequence alignment of cDNA subclone 008#10 with mouse Hpcl by BLAST searching with the National Center for Biotechnology Information database --- p.371 / Chapter C26.3 --- Summary of sequence alignment of cDNA subclone 008#l0 with mouse Hpcl --- p.371 / Chapter C27.1 --- DNA sequence of cDNA subclone 009#6 using Ml3 forward -20 primer and the 3´ة-AP9 --- p.372 / Chapter C27.2 --- Sequence alignment of cDNA subclone 009#6 with mouse Hpcl by BLAST searching with the National Center for Biotechnology Information database --- p.372 / Chapter C27.3 --- Summary of sequence alignment of cDNA subclone 009#6 with mouse Hpcl --- p.372 / Chapter C28.1 --- DNA sequence of cDNA subclone 009#8 using Ml3 reverse primer and the sequence of 3'-AP9 --- p.373 / Chapter C28.2 --- Sequence alignment of cDNA subclone 009#8 with mouse Hpcl by BLAST searching with the National Center for Biotechnology Information database --- p.373 / Chapter C28.3 --- Summary of sequence alignment of cDNA subclone 009#8 with mouse Hpcl --- p.373 / Chapter C29.1 --- DNA sequence of cDNA subclone PP3#3 using M13 forward -20 primer and the sequence of 5'-ARP3 --- p.374 / Chapter C29.2 --- Sequence alignment of cDNA subclone PP3#3 with mouse CYP4A14 by BLAST searching with the National Center --- p.374 / Chapter C29.3 --- Summary of sequence alignment of cDNA subclone PP3#3 with mouse CYP4A14 --- p.374 / Chapter C30.1 --- DNA sequence of cDNA subclone PP3#13 using Ml3 reverse primer and the sequence of 5'-ARP3 --- p.375 / Chapter C30.2 --- Sequence alignment of cDNA subclone PP3#13 with mouse CYP4A14 by BLAST searching with the National Center for Biotechnology Information database --- p.375 / Chapter C30.3 --- Summary of sequence alignment of cDNA subclone PP3#13 with mouse CYP4A14 --- p.375 / Chapter C31.1 --- DNA sequence of cDNA subclone QQ4#14 using Ml3 forward -20 primer and the sequence of 5´ة-ARP2 --- p.376 / Chapter C31.2 --- Sequence alignment of cDNA subclone QQ4#14 with mouse MCAD by BLAST searching with the National Center for Biotechnology Information database --- p.376 / Chapter C31.3 --- Summary of sequence alignment of cDNA subclone QQ4#14 with mouse MCAD --- p.376 / Chapter C32.1 --- DNA sequence of cDNA subclone QQ4#15 using Ml3 reverse primer and the sequence of 5´ة-ARP2 --- p.377 / Chapter C32.2 --- Sequence alignment of cDNA subclone QQ4#15 with mouse MCAD by BLAST searching with the National Center for Biotechnology Information database --- p.377 / Chapter C32.3 --- Summary of sequence alignment of cDNA subclone QQ4#15 with mouse MCAD --- p.377 / Chapter C33.1 --- DNA sequence of cDNA subclone QQ5#14 using Ml3 forward -20 primer and the sequence of 3'-AP3 --- p.377 / Chapter C33.2 --- Sequence alignment of cDNA subclone QQ5#14 with mouse MUP II by BLAST searching with the National Center for Biotechnology Information database --- p.378 / Chapter C33.3 --- Summary of sequence alignment of cDNA subclone QQ5#14 with mouse MUP II --- p.378 / Chapter C34.1 --- DNA sequence of cDNA subclone QQ5#17 using Ml3 reverse primer and the sequence of 5'-ARP2 --- p.378 / Chapter C34.2 --- Sequence alignment of cDNA subclone QQ5#17 with mouse MUP II by BLAST searching with the National Center for Biotechnology Information database --- p.379 / Chapter C34.3 --- Summary of sequence alignment of cDNA subclone QQ5#17 with mouse MUP II --- p.379 / Chapter C35.1 --- DNA sequence of cDNA subclone QQ6#13 using Ml3 forward -20 primer and the sequence of 3'-AP3 --- p.380 / Chapter C35.2 --- Sequence alignment of cDNA subclone QQ6#13 with mouse MUP II by BLAST searching with the National Center for Biotechnology Information database --- p.380 / Chapter C35.3 --- Summary of sequence alignment of cDNA subclone QQ6#13 with mouse MUP II --- p.380 / Chapter C36.1 --- DNA sequence of cDNA subclone QQ6#14 using M13 reverse primer and the 5'-ARP2 --- p.381 / Chapter C36.2 --- Sequence alignment of cDNA subclone QQ6#14 with mouse MUP II by BLAST searching with the National Center for Biotechnology Information database --- p.381 / Chapter C36.3 --- Summary of sequence alignment of cDNA subclone QQ6#14 with mouse MUP II --- p.381 / Chapter C37.1 --- DNA sequence of cDNA subclone TT4#5 using Ml3 forward -20 primer and the sequence of 3´ة-AP5 --- p.382 / Chapter C37.2 --- Sequence alignment of cDNA subclone TT4#5 with mouse MUP II by BLAST searching with the National Center for Biotechnology Information database --- p.382 / Chapter C37.3 --- Summary of sequence alignment of cDNA subclone TT4#5 with mouse MUP II --- p.382 / Chapter C38.1 --- DNA sequence of cDNA subclone TT4#8 using Ml3 forward -20 primer and the sequence of 5´ة-ARP2 --- p.383 / Chapter C38.2 --- Sequence alignment of cDNA subclone TT4#8 with mouse MUP II by BLAST searching with the National Center for Biotechnology Information database --- p.383 / Chapter C38.3 --- Summary of sequence alignment of cDNA subclone TT4#8 with mouse MUP II --- p.383 / Chapter C39.1 --- DNA sequence of cDNA subclone TT4#9 using Ml3 reverse primer and the sequence of 3'-AP5 --- p.384 / Chapter C39.2 --- Sequence alignment of cDNA subclone TT4#9 with mouse MUP II by BLAST searching with the National Center for Biotechnology Information database --- p.384 / Chapter C39.3 --- Summary of sequence alignment of cDNA subclone TT4#9 with mouse MUP II --- p.384 / Chapter C40.1 --- DNA sequence of cDNA subclone TT5#1 using Ml3 forward -20 primer and the sequence of 5'-ARP2 --- p.385 / Chapter C40.2 --- Sequence alignment of cDNA subclone TT5#1 with mouse MUP II by BLAST searching with the National Center for Biotechnology Information database --- p.385 / Chapter C40.3 --- Summary of sequence alignment of cDNA subclone TT5#1 with mouse MUP II --- p.385 / Chapter C41.1 --- DNA sequence of cDNA subclone TT5#9 using Ml3 reverse primer and the sequence of 3´ة-AP5 --- p.386 / Chapter C41.2 --- Sequence alignment of cDNA subclone TT5#9 with mouse MUP II by BLAST searching with the National Center for Biotechnology Information database --- p.386 / Chapter C41.3 --- Summary of sequence alignment of cDNA subclone TT5#9 with mouse MUP II --- p.386 / Chapter C42.1 --- DNA sequence of cDNA subclone UU1#1 using Ml3 forward -20 primer and the sequence of 3´ة-AP2 --- p.387 / Chapter C42.2 --- Sequence alignment of cDNA subclone UU1#1 with mouse CYP4A10 by BLAST searching with the National Center for Biotechnology Information database --- p.387 / Chapter C42.3 --- Summary of sequence alignment of cDNA subclone UU1#1 with mouse CYP4A10 --- p.387 / Chapter C43.1 --- DNA sequence of cDNA subclone UU1#4 using Ml3 reverse primer and the sequence of 5'-ARP12 --- p.388 / Chapter C43.2 --- Sequence alignment of cDNA subclone UU1#4 with mouse CYP4A10 by BLAST searching with the National Center for Biotechnology Information database --- p.388 / Chapter C43.3 --- Summary of sequence alignment of cDNA subclone UU1#4 with mouse CYP4A10 --- p.388 / Chapter C44.1 --- DNA sequence of cDNA subclone VV5#2 using Ml3 forward -20 primer and the sequence of 3´ة-AP2 --- p.389 / Chapter C44.2 --- Sequence alignment of cDNA subclone VV5#2 with mouse CYP4A10 by BLAST searching with the National Center for Biotechnology Information database --- p.389 / Chapter C44.3 --- Summary of sequence alignment of cDNA subclone VV5#2 with mouse CYP4A10 --- p.389 / Chapter C45.1 --- DNA sequence of cDNA subclone VV5#3 using Ml3 forward -20 primer and the sequence of 3'-AP2 --- p.390 / Chapter C45.2 --- Sequence alignment of cDNA subclone VV5#3 with mouse CYP4A10 by BLAST searching with the National Center for Biotechnology Information database --- p.390 / Chapter C45.3 --- Summary of sequence alignment of cDNA subclone VV5#3 with mouse CYP4A10 --- p.390 / Chapter C46.1 --- DNA sequence of cDNA subclone VV5#4 using Ml3 reverse primer and the sequence of 5'-ARP18 --- p.391 / Chapter C46.2 --- Sequence alignment of cDNA subclone VV5#4 with mouse CYP4A10 by BLAST searching with the National Center for Biotechnology Information database --- p.391 / Chapter C46.3 --- Summary of sequence alignment of cDNA subclone VV5#4 with mouse CYP4A10 --- p.391 / Chapter C47.1 --- DNA sequence of cDNA subclone VV8#5 using M13 forward -20 primer and the sequence of 5'-ARP18 --- p.392 / Chapter C47.2 --- Sequence alignment of cDNA subclone VV8#5 with mouse HMG-CoAS by BLAST searching with the National Center for Biotechnology Information database --- p.392 / Chapter C47.3 --- Summary of sequence alignment of cDNA subclone VV8#5 with mouse HMG-CoAS --- p.392 / Chapter C48.1 --- DNA sequence of cDNA subclone VV8#6 using Ml 3 reverse primer and the sequence of 3'-AP2 --- p.393 / Chapter C48.2 --- Sequence alignment of cDNA subclone VV8#6 with mouse HMG-CoAS by BLAST searching with the National Center for Biotechnology Information database --- p.393 / Chapter C48.3 --- Summary of sequence alignment of cDNA subclone VV8#6 with mouse HMG-CoAS --- p.393 / Chapter C49.1 --- DNA sequence of cDNA subclone WW1#3 using Ml 3 forward -20 primer and the sequence of 3'-AP2 --- p.394 / Chapter C49.2 --- Sequence alignment of cDNA subclone WW1#3 with mouse Spil-3 by BLAST searching with the National Center for Biotechnology Information database --- p.394 / Chapter C49.3 --- Summary of sequence alignment of cDNA subclone WW1#3 with mouse Spil-3 --- p.394 / Chapter C50.1 --- DNA sequence of cDNA subclone WW1#4 using Ml3 forward -20 primer and the sequence of 3'-AP2 --- p.395 / Chapter C50.2 --- Sequence alignment of cDNA subclone WW1#4 with mouse Spil-3 by BLAST searching with the National Center for Biotechnology Information database --- p.395 / Chapter C50.3 --- Summary of sequence alignment of cDNA subclone WW1#4 with mouse Spil-3 --- p.395 / Chapter C51.1 --- DNA sequence of cDNA subclone WW1#7 using M13 reverse primer and the sequence of 5´ة-ARP19 --- p.396 / Chapter C51.2 --- Sequence alignment of cDNA subclone WW1#7 with mouse Spil-3 by BLAST searching with the National Center for Biotechnology Information database --- p.396 / Chapter C51.3 --- Summary of sequence alignment of cDNA subclone WW1#7 ith mouse Spil-3 --- p.396 / Chapter C52.1 --- DNA sequence of cDNA subclone XX1#2 using Ml3 forward 20 primer and the sequence of 3´ة-APl0 --- p.397 / Chapter C52.2 --- Sequence alignment of cDNA subclone XX1#2 with mouse CYP4A10 by BLAST searching with the National Center for Biotechnology Information database --- p.397 / Chapter C52.3 --- Summary of sequence alignment of cDNA subclone XX1#2 with mouse CYP4A10 --- p.397 / Chapter C53.1 --- DNA sequence of cDNA subclone XX1#3 using M13 forward -20 primer and the sequence of 3´ة-APl0 D205 --- p.398 / Chapter C53.2 --- Sequence alignment of cDNA subclone XX1#3 with mouse CYP4A10 by BLAST searching with the National Center for Biotechnology Information database --- p.398 / Chapter C53.3 --- Summary of sequence alignment of cDNA subclone XX1#3 with mouse CYP4A10 --- p.398 / Chapter C54.1 --- DNA sequence of cDNA subclone XX1#9 using Ml3 forward -20 primer and the sequence of 5'-ARP12 --- p.399 / Chapter C54.2 --- Sequence alignment of cDNA subclone XX1#9 with mouse CYP4A10 by BLAST searching with the National Center for Biotechnology Information database --- p.399 / Chapter C54.3 --- Summary of sequence alignment of cDNA subclone XX1#9 with mouse CYP4A10 --- p.399
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324418 |
| Date | January 2003 |
| Contributors | Lo, Kam Chun., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xliv, 399 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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