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Expansion and Osteogenic Differentiation of Human Umbilical Cord Perivascular Stem Cells by Low Intensity Pulsed Ultrasound for Dentofacial Tissue Engineering

The objective of these experiments is to explore the effect of LIPUS on the ultraexpansion and osteogenic differentiation of harvested passage-4 HUCPV-SCs. HUCPV-SCs were divided into two groups: a treatment group that received LIPUS for 10 minutes for 1, 7, and 14 days and a control group that received a sham treatment utilizing both basic and osteogenic media. The results in basic media and osteogenic media demonstrated nonsignificant differences in cell count, ALP, DNA content, and CD90. Statistically significant expression of OSP and PCNA was observed on day 14 in LIPUS treated group. Nucleostemin expression in the LIPUS-treated group was insignificant on days 1 and 7. However, a selective increase in osteogenic markers was obtained on day 7 for ALP and OCN and on day 14 for OPN. Future experiments are required to explore the effects of different application times and/or techniques of LIPUS on the behaviour of HUCPV-SCs. / Medical Science

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:AEU.10048/741
Date11 1900
CreatorsAldosary, Tagreed
ContributorsEl-Bialy, Tarek (Orthodontics), Uludag, Hasan (Chemical Engineering), Doschak, Michael (Pharmacy), El-Bialy, Tarek (Orthodontics), Uludag, Hasan (Chemical Engineering), Doschak, Michael (Pharmacy), Eggert, Michael (Periodontics)
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeThesis
Format16926941 bytes, application/pdf

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