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Molekulární identifikace vybraných druhů bakterií mléčného kvašení a bifidobakterií v doplňcích stravy / Molecular identification of selected species of lactic acid bacteria and bifidobacteria in food additives

Probiotic lactic acid bacteria and bifidobacteria are natural part of microflora of gastrointestinal tract. In the present, day they are grossly exploited in food processing industry. The aim of the work was molecular identification of bacteria of genus Lactobacillus and Bifidobacterium in complex matrices of two food additives. Total DNA was isolated from crude cell lysates by magnetic particles P(HEMA-co-GMA). Isolated DNA was amplified in genus-specific and species-specific PCRs. Amplicons were detected by agarose gel electrophoresis. Results were compared with declared specification given by producers in three different batches.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:216692
Date January 2011
CreatorsRiegelová, Kristýna
ContributorsRittich, Bohuslav, Španová, Alena
PublisherVysoké učení technické v Brně. Fakulta chemická
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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