Reconstruction of bone is needed for high bone loss due to congenital deformities,
trauma or neoplastic diseases. Commonly, orthopaedic surgical treatments are
autologus or allogenic bone implant or prosthetic implant. A choice to the traditional
approaches could be represented by tissue engineering that use cells (and/or their
products) and innovative biomaterials to perform bone substitutes biologically active
as an alternative to artificial devices. In the last years, there was a wide improvement
in biology on stem cells potential research and in biomedical engineering through
development of new biomaterials designed to resemble the physiological tissues.
Tissue engineering strategies and smart materials aim together to stimulate in vivo
bone regeneration. This approaches drive at restore not only structure integrity and/or
function of the original tissue, but also to induce new tissue deposition in situ. An
intelligent bone substitute is now designed like not only a scaffold but also as carrier
of regeneration biomolecular signals. Biomimetics has helped to project new tissue
engineered devices to simulate the physiological substrates architecture, such
extracellular matrix (ECM), and molecular signals that drive the integration at the
interface between pre-existing tissue and scaffold. Biomimetic strategies want to
increase the material surface biological activity with physical modifications
(topography) o chemical ones (adhesive peptides), to improve cell adhesion to
material surface and possibly scaffold colonization.
This study evaluated the effects of biomimetic modifications of surgical materials
surface, as poly-caprolattone (PCL) and titanium on bone stem cells behaviour in a
marrow experimental model in vitro. Two biomimetic strategies were analyzed; ione
beam irradiation, that changes the surface roughness at the nanoscale, and surface
functionalization with specific adhesive peptides or Self Assembled Monolayers
(SAMs). These new concept could be a mean to improve the early (cell adhesion,
spreading..) and late phases (osteoblast differentiation) of cell/substrate interactions.
| Identifer | oai:union.ndltd.org:unibo.it/oai:amsdottorato.cib.unibo.it:705 |
| Date | 15 May 2008 |
| Creators | Devescovi, Valentina <1975> |
| Contributors | Giunti, Armando |
| Publisher | Alma Mater Studiorum - Università di Bologna |
| Source Sets | Università di Bologna |
| Language | Italian |
| Detected Language | English |
| Type | Doctoral Thesis, PeerReviewed |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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