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Some studies on the effects of oestrogens on mast cells.

Law, Ka Yan Jessica. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 194-203). / Abstracts in English and Chinese. / Abstract (English) --- p.i / Abstract (Chinese) --- p.iv / Acknowledgements --- p.vi / Publications --- p.vii / Abbreviations --- p.viii / Chapter 1. --- Introduction --- p.1 / Chapter 1.1 --- Mast Cell --- p.1 / Chapter 1.1.1 --- Origin of Mast Cell --- p.2 / Chapter 1.1.2 --- Heterogeneity of Mast Cell --- p.3 / Chapter 1.1.3 --- Activation of Mast Cells --- p.5 / Chapter 1.1.3.1 --- IgE-dependent Mast cell Activation --- p.5 / Chapter 1.1.3.2 --- IgE-independent Mast Cell Activation --- p.10 / Chapter 1.1.3.3 --- Importance of Calcium --- p.12 / Chapter 1.1.3.4 --- "Role of cyclic adenosine ´3ة,5'-monophosphate (cAMP)" --- p.15 / Chapter 1.1.4 --- Mast Cells Mediators --- p.16 / Chapter 1.1.5 --- Physiological and Pathological Roles of Mast Cells --- p.19 / Chapter 1.2 --- Oestrogens --- p.22 / Chapter 1.2.1 --- Oestrogens and Inflammation --- p.23 / Chapter 1.2.1.1 --- Pro-inflammatory Effects of Oestrogen --- p.23 / Chapter 1.2.1.2 --- Anti-inflammatory Effects of Oestrogen --- p.24 / Chapter 1.2.2 --- Oestrogen Signaling --- p.25 / Chapter 1.2.2.1 --- Classical Genomic Pathway --- p.26 / Chapter 1.2.2.2 --- Non-genomic Pathway --- p.30 / Chapter 1.3 --- Aims of Study --- p.34 / Chapter 2. --- Materials and Methods --- p.36 / Chapter 2.1 --- Materials --- p.36 / Chapter 2.1.1 --- Drugs --- p.36 / Chapter 2.1.1.1 --- Oestrogen Receptor Agonists and Antagonists --- p.36 / Chapter 2.1.1.2 --- DNA Transcription and Translation Blockers --- p.41 / Chapter 2.1.1.3 --- Mast Cell Secretagogues --- p.41 / Chapter 2.1.2 --- Materials for Histamine Assay Experiments --- p.41 / Chapter 2.1.2.1 --- Materials for Rat Sensitization --- p.41 / Chapter 2.1.2.2 --- Materials for Ovariectomy surgery --- p.42 / Chapter 2.1.2.3 --- Materials for Buffers --- p.42 / Chapter 2.1.2.4 --- Materials for Histamine Release Assay --- p.43 / Chapter 2.1.2.5 --- Miscellaneous --- p.43 / Chapter 2.1.3 --- Materials for Oestrogen Receptors Identification --- p.44 / Chapter 2.1.3.1 --- Oestrogen Receptors Expression --- p.44 / Chapter 2.1.3.1.1 --- Materials for RNA Extraction --- p.44 / Chapter 2.1.3.1.2 --- Materials for cDNA Construction by RT- PCR --- p.45 / Chapter 2.1.3.1.3 --- Materials for PCR --- p.45 / Chapter 2.1.3.1.4 --- Materials for Agarose Gel Electrophoresis --- p.46 / Chapter 2.1.3.1.5 --- Miscellaneous --- p.46 / Chapter 2.2 --- Histamine Assay Experiments --- p.47 / Chapter 2.2.1 --- Preparation of Buffers --- p.47 / Chapter 2.2.2 --- Preparation of Stock Solutions --- p.48 / Chapter 2.2.2.1 --- Preparation of Mast Cell Secretagogues Stock Solutions --- p.48 / Chapter 2.2.2.2 --- Preparation of Oestrogens Stock Solutions --- p.48 / Chapter 2.2.3 --- Animals and Rat Peritoneal Mast Cell Isolation --- p.49 / Chapter 2.2.3.1 --- Animals --- p.49 / Chapter 2.2.3.2 --- Rats Sensitization --- p.49 / Chapter 2.2.3.3 --- Ovariectomy Surgery --- p.49 / Chapter 2.2.3.4 --- Isolation of Rat Peritoneal Mast Cells --- p.50 / Chapter 2.2.3.5 --- Purification of Mast Cells --- p.51 / Chapter 2.2.3.6 --- Determination of Mast Cell Number and Viability --- p.51 / Chapter 2.2.4 --- Histamine Release Assay and Histamine Measurement --- p.52 / Chapter 2.2.4.1 --- Histamine Release Assay --- p.52 / Chapter 2.2.4.2 --- Fluorescence Assay of Histamine --- p.53 / Chapter 2.2.5 --- Calculation of Histamine Release Level and Inhibition --- p.54 / Chapter 2.2.6 --- Statistics Analysis --- p.55 / Chapter 2.3 --- Oestrogen Receptors Identification --- p.55 / Chapter 2.3.1 --- Oestrogen Receptors Expression --- p.55 / Chapter 2.3.1.1 --- Preparation of Buffers --- p.55 / Chapter 2.3.1.2 --- Isolation of Mast Cell mRNA --- p.56 / Chapter 2.3.1.3 --- Heparinase Treatment --- p.57 / Chapter 2.3.1.4 --- Construction of Mast Cell cDNA by RT-PCR --- p.57 / Chapter 2.3.1.5 --- Amplification of Oestrogen receptors cDNA by PCR method --- p.58 / Chapter 2.3.1.6 --- Agarose Gel Electrophoresis --- p.58 / Chapter 3. --- Effects of Oestrogens on Immunological Induced Histamine Release from Rat Peritoneal Mast Cells --- p.59 / Chapter 3.1 --- Introduction --- p.59 / Chapter 3.2 --- Materials and Methods --- p.62 / Chapter 3.3 --- Results --- p.63 / Chapter 3.3.1 --- Activation of RPMCs by Immunological Stimuli --- p.63 / Chapter 3.3.1.1 --- Effects of Oestrogen Receptor Agonists on Histamine Release from RPMCs --- p.63 / Chapter 3.3.1.2 --- Effects of Oestrogen on Anti-IgE mediated Histamine Release from RPMCs --- p.64 / Chapter 3.3.1.3 --- Effects of Selective Oestrogen Receptor Modulators on Anti-IgE Mediated Histamine Release from RPMCs --- p.65 / Chapter 3.3.1.4 --- Effects of Selective Oestrogen Agonists on Anti- IgE Mediated Histamine Release from RPMCs --- p.67 / Chapter 3.4 --- Discussion --- p.69 / Chapter 4. --- Elucidating the Mechanism Underlying the Effects of Oestrogens on Rat Peritoneal Mast Cells --- p.110 / Chapter 4.1 --- Introduction --- p.110 / Chapter 4.2 --- Materials and Methods --- p.115 / Chapter 4.3 --- Results --- p.116 / Chapter 4.3.1 --- The Involvement of Membrane Oestrogen Receptors --- p.117 / Chapter 4.3.1.1 --- Effects of Membrane Impermeable Oestrogen on Anti-IgE mediated Histamine Release from RPMCs --- p.117 / Chapter 4.3.2 --- Effects of Oestrogen Receptor Antagonists on the Modulating Actions of Oestrogen Receptor Agonists and SERMs on RPMC of Male Rats --- p.118 / Chapter 4.3.2.1 --- Effects on Oestrogen Receptor Agonists and SERMs Induced Low Level Histamine Release from RPMCs --- p.118 / Chapter 4.3.2.2 --- Effects on the Inhibitory Actions on Anti-IgE Mediated Histamine Release from RPMCs --- p.118 / Chapter 4.3.3 --- Effects of Transcription and Translation Blockers on the Inhibition Effects of Oestrogen Receptor Agonists and SERMs on RPMC of Male Rats --- p.122 / Chapter 4.3.4 --- Identification of Oestrogen Receptor Subtypes on RPMCs --- p.123 / Chapter 4.3.4.1 --- Molecular Expression of Oestrogen Receptor Subtypes on RPMCs --- p.123 / Chapter 4.3.5 --- Effects of Oestrogenic Agents on activation of RPMCs by Non- Immunological Stimuli --- p.123 / Chapter 4.3.5.1 --- Effects of Oestrogen on Compound48/80 Mediated Histamine Release from RPMCs --- p.123 / Chapter 4.3.5.2 --- Effects of Selective Oestrogen Receptor Modulators on Compound 48/80 Mediated Histamine Release from RPMCs --- p.124 / Chapter 4.3.5.3 --- Effects of Membrane Impermeable Oestrogen on Compound 48/80 Mediated Histamine Release from RPMCs --- p.125 / Chapter 4.3.5.4 --- Effects of Oestrogen on Substance P Mediated Histamine Release from RPMCs --- p.125 / Chapter 4.3.5.5 --- Effects of Selective Oestrogen Receptor Modulators on Substance P Mediated Histamine Release from RPMCs --- p.126 / Chapter 4.3.5.6 --- Effects of Membrane Impermeable Oestrogen on Substance P Mediated Histamine Release from RPMCs / Chapter 4.4 --- Discussion --- p.128 / References --- p.194

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326209
Date January 2007
ContributorsLaw, Ka Yan Jessica., Chinese University of Hong Kong Graduate School. Division of Pharmacology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xiii, 203 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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