The archaeal branch of life represents some of the oldest life forms on Earth. Archaea are believed to have diverged from Prokaryotes roughly 3.5 billion years ago and it’s theorized that biological methane production started around this time as well. This would make methanogenesis one of the oldest metabolisms on our planet. Methanogenesis is a process that, so far, is known to be unique to archaea. Since their evolution, methanogens have had massive impacts on Earth’s climate and biology. Methane is an important part of the global carbon cycle, but is also a major greenhouse gas, making it a vital area of research. The methanogen studied in this thesis is referred to as the wild type (WT) and was isolated from an active serpentinization system at Chimaera seep in Çıralı, Antalya Gulf, Turkey. The Chimaera seep is a geological formation analogous to mid-ocean ridges, but exposed and above land. The research in this thesis focuses on the description of the WT, which is believed to be a new species. Describing the WT consisted of the characterization of extremes as well as optimal growth conditions. The WT was found to grow at initial pH levels of 9.0 and 9.5. It grew from 15 °C to 45 °C, but not at 47 °C and not at 12 °C, and had an optimum at 39 °C. The WT had measurable growth up to 40 g/L NaCl, and had its optimum at 0 g/L. The WT grew best with H2/CO2 substrate, but also grew well on formate.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-511280 |
| Date | January 2023 |
| Creators | Stephens, Aubree |
| Publisher | Uppsala universitet, Institutionen för biologisk grundutbildning, Department of Molecular Biology, Swedish University of Agricultural Sciences |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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