Yes / Multiple factors and conditions can lead to impaired wound healing. Chronic non-healing wounds
are a common problem among the elderly. To identify microRNAs negatively impacting the wound
repair, global miRNA profiling of wounds collected from young and old mice was performed. A
subset of miRNAs that exhibited an age-dependent expression pattern during wound closure was
identified, including miR-31 and miR-200c. The expression of miR-200 family members was markedly
downregulated upon wounding in both young and aged mice, with an exception of acute
upregulation of miR-200c at the early phase of wound healing in aged skin. In unwounded aged skin
(versus unwounded younger skin), the level of miR-200c was also found elevated in both human and
mice. Overexpression of miR-200c in human ex vivo wounds delayed re-epithelialisation and
inhibited cell proliferation in the wound epithelium. Modulation of miR-200c expression in both
human and mouse keratinocytes in vitro revealed inhibitory effects of miR-200c on migration, but
not proliferation. Accelerated wound closure in vitro induced by anti-miR-200c was associated with
upregulation of genes controlling cell migration. Thus, our study identified miR-200c as a critical
determinant that inhibits cell migration during skin repair after injury and may contribute to ageassociated
alterations in wound repair. / Supported by a grant from Medical Research Council UK (MR/K011324/1)
| Identifer | oai:union.ndltd.org:BRADFORD/oai:bradscholars.brad.ac.uk:10454/12063 |
| Date | 12 June 2017 |
| Creators | Aunin, Eerik, Broadley, David, Ahmed, Mohammed I., Mardaryev, Andrei N., Botchkareva, Natalia V. |
| Source Sets | Bradford Scholars |
| Language | English |
| Detected Language | English |
| Type | Article, Published version |
| Rights | © 2017 The Authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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