In conclusion, the results suggested the important role of Ncl in driving the optic vesicle formation during early eye development. / The eye is a complex sense organ. It develops from different embryonic origins that including neural ectoderm, surface ectoderm, neural crest and paraxial mesoderm. Morphogenetic waves occur during eye development involve timely interactions of transcription factors and inductive signaling to ensure the correct temporal and spatial development of different components. Genetic studies of congenital eye defects, especially mutation screening and gene targeting, have provided the information about the molecular regulation in the complex processes of eye development. However, our knowledge of the basic genetic pathways that regulate the normal embryonic eye formation is incomplete. / Though the developing eye is believed to be highly specialized extension from the developing neural tube, the formation of major eye structure involves independent coordination of inductive interactions and regional specifications; formation of neural connections between retina and optic tectum; and maturation to a functional eye. There is not much information about eye-specific expression in early embryonic period. In this study, microarray was used to profile the molecular changes occurring in the developing mouse eye between the stage of optic vesicle evagination at E9.5 and completion of basic eye formation at P0. Differentially expressed transcription factor and signaling molecules, including nucleolin gene (Ncl), in the early developing eye were displayed. Temporal expression patterns were confirmed by quantitative real time PCR and spatial expressions patterns were confirmed by the whole-mount in situ hybridization. siRNA and overexpression vector targeting nucleolin transcript was designed to study their roles in the early eye morphogenesis during mouse embryogenesis in vitro. The loss of function phenotype after nucleolin knockdown was demonstrated by the absence of early optic vesicles with normal neural tube in the developing mouse embryos. Ectopic optic vesicle in developing mouse embryo was resulted under overexpression of Ncl . With the aim to study the biological roles of Ncl in mouse embryonic eye development in vivo, both conventional and conditional knockout techniques were attempted. The expression and functional studies revealed that a new neural tube independent signaling pathway regulated in the induction and formation of optic vesicles in the early eye formation. / Tang, Ling Yin. / Source: Dissertation Abstracts International, Volume: 70-06, Section: B, page: 3294. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 138-146). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstracts in English and Chinese. / School code: 1307.
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_344214 |
| Date | January 2008 |
| Contributors | Tang, Ling Yin., Chinese University of Hong Kong Graduate School. Division of Obstetrics and Gynaecology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, theses |
| Format | electronic resource, microform, microfiche, 1 online resource (xxi, 146 leaves : ill.) |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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