Bibliography: pages 135-150. / This dissertation represents a continuation of the research on the sucrose utilization system of the aerobic, collagenolytic, halotolerant, Gram-negative bacterium Vibrio alginolyticus. The V. alginolyticus sucrose utilization system originally cloned into Escherichia coli on plasmid pVS100 involves a sucrase enzyme (gene scrB), and a sucrose uptake system. Synthesis of the sucrase and sucrose uptake system in V. alginolyticus and E. coli(pVS100) is regulated. The nucleotide sequence and analysis of DNA regions encoding the sucrose uptake and regulatory functions are presented here. An investigation of the expression of the. V. alginolyticus sucrose utilization system in Bacillus subtilis is also presented.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uct/oai:localhost:11427/22110 |
| Date | January 1990 |
| Creators | Blatch, Gregory Lloyd |
| Contributors | Woods, David R |
| Publisher | University of Cape Town, Faculty of Science, Department of Molecular and Cell Biology |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Doctoral Thesis, Doctoral, PhD |
| Format | application/pdf |
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