Bibliography : pages 234-264. / Some genetic systems operative in Bacteroides fragilis have been successfully investigated. Firstly the bacteriocin produced by the B.fragilis BF-1 strain was purified and partially characterized. This bacteriocin was found to be cell bound and constitutively produced by the bacteria. The purified bacteriocin was a protein with an apparent Mr of 6400-7200, and was relatively heat stable. The action of the bacteriocin resulted in the lysis of sensitive bacteria. As previous reports indicated that a bacteriocin isolated from the BF-1 strain inhibited the action of RNA-polymerase in-vivo and in-vitro, RNA-polymerase was extracted and partially purified from the bacteriocin sensitive B.fragilis BF-2 strain (described in Appendix 5). This enzyme was essentially similar to that described for other Eubacteriales, but was not inhibited in the in vitro assay by the purified bacteriocin described in this thesis.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uct/oai:localhost:11427/21909 |
| Date | January 1986 |
| Creators | Southern, James Arnold |
| Contributors | Woods, David R |
| Publisher | University of Cape Town, Faculty of Science, Department of Molecular and Cell Biology |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Doctoral Thesis, Doctoral, PhD |
| Format | application/pdf |
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