Includes bibliographical references. / Eleven bacterial strains that have been isolated from the ostrich hindgut, have successfully been identified with the aid of PCR amplification of the 16S rDNA genes and sequence analysis using the BLASTN search of the GenBank database. The nucleotide sequences were determined either through direct sequencing of the PCR products or after cloning the products into the plasmid vector pBluescript (SK). The results obtained from the 16S rDNA sequence homologies strongly suggests that the newly identified bacteria should be assigned to three major bacterial genera, namely, Ruminococcus, Buryrivibrio and Bacteroides. Four highly cellulolytic strains were identiļ¬ed as ruminococci while two weakly cellulolytic strains, showed highest homology to members of the genus Butyrivibrio. Another weakly cellulolytic strain showed closest identity to the sulfur reducing bacterial genus Desulfovibrio. Four non-cellulolytic strains showed highest homology to the genus Bacteroides.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uct/oai:localhost:11427/9740 |
| Date | January 2000 |
| Creators | Russouw, Tracy Karen Joe |
| Contributors | Thomson, Jennifer Ann, Reid, Sharon J |
| Publisher | University of Cape Town, Faculty of Science, Department of Molecular and Cell Biology |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Master Thesis, Masters, MPhil |
| Format | application/pdf |
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