smig-1(ev809) is a novel suppressor of multiple distal tip cell (DTC) migration mutants in the nematode Caenorhabditis elegans. In the C. elegans hermaphrodite, the two U-shaped gonad arms develop and form as a result of the migration of two DTCs. smig-1(ev809) suppresses DTC migration defects of mutants encoding components of intracellular glycosylation pathways as well as extracellular basement membrane glycoproteins. The smig-1(ev809) mutation bypasses the requirement for a fully functional chondroitin pathway and MIG-17 metalloprotease in DTC pathfinding. I found that i) suppression of the hypomorphic chondroitin mutant mig-22(k141) is not completely dependent on MIG-17 activity; ii) the smig-1(ev809) mutant is likely to be a loss- of-function suppressor; and iii) SMIG-1 does not visibly affect the localization of poorly glycosylated MIG-17 on the gonad surface. Understanding SMIG-1 function will shed light on the role of glycosylation, the extracellular matrix and basement membranes in cell migration during development.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/44068 |
| Date | 19 March 2014 |
| Creators | Tran, Nhat |
| Contributors | Culotti, Joseph |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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