The dissertation deals with the risk of clostridia presence in animal feed, particularly in silages, excrements, and in litter (separate of cattle slurry). At the same time, their presence was observed in the rumen fluid and milk. The main objective of the study was to assess the effect of addition of surface layer of soil in different levels of contamination (0, 20, 40 g/kg of dry matter) and addition of silage additives on hygienic quality of silage, especially on the presence of clostridia and on microorganisms forming spores in the experimental model silages and in operating conditions. Microorganisms forming spores were assessed in all samples. Their counts increased with the higher addition of topsoil. Clostridium perfringens was identified using PCR in silage made of wilted clover, in ruminal fluid (the enterprise A), in excrements coming from both operating conditions (A, B), and separate of cattle slurry coming from the enterprise B. At the same time, positive effect of biological and chemical additives on silage reducing the number of undesirable microorganisms was confirmed.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:425079 |
| Date | January 2016 |
| Creators | Mlejnková, Veronika |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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