The diploma thesis is focused on the verification of inheritance of selected microsatellites from the microsatellite panel of the MHC locus in camels. The theoretical part deals with the characteristics of the genus Camelus. Special focus is given to adaptation mechanisms allowing camels to survive in conditions that are inhospitable for other livestock species. Microsatellites which are also described became an excellent choice not only in parentage analysis. The practical part was based on fragment analysis in genetic analyser ABI PRISM 3500, which preceded DNA isolation, multiplex PCR amplification and gel electrophoresis. Using GeneMapper 5 software, amplified fragment sizes were calculated and mendelian segregation verified. For assembly microsatellite panel four microsatellite markers were selected, M29_I, M35_II, M41_III a M42_III. The least polymorphic was microsatellite M29_I of the MHC I region and the most polymorphic was microsatellite M42_III of the MHC III region. In general, microsatellites from the microsatellite panel can be considered reliable for verifying the origin of the alleles and can be further used to reveal polymorphism of the MHC region of camels.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:427668 |
| Date | January 2019 |
| Creators | Grygarová, Tereza |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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