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Effect of k-casein glycosylation on the properties of milk

A study was conducted to determine the relationship between the degree of glycosylation of $ kappa$-casein (CN) and physicochemical properties of milk: heat stability and ethanol stability. Morning milk samples from individual Holstein cows (genotype $ kappa$-CN AA, $ beta$-CN A$ sb1$A$ sb2$, $ alpha sb{ rm s1}$-CN BB, $ beta$-lactoglobulin AB) in midlactation were collected and analyzed for protein and somatic cell count. Samples of low somatic cell count ($<$150000 cells/ml) were skimmed and dialyzed overnight at 4$ sp circ$C against bulk tank milk to equilibrate soluble components. Three series of analyses were performed for the determination of thermostability of milk between pH 6.5 and 9. In the first 2 sections (A and B), the heat clotting time vs pH (HCT/pH) profiles of normal milk were measured. The effect of sialic acid (NANA) depletion on the HCT/pH profile was tested in a third series of experiments. And finally, a series of analyses were performed to determine the ethanol stability of milk. (Abstract shortened by UMI.)

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.26234
Date January 1994
CreatorsAyers, Carolyn
ContributorsRobitaille, Gilles (advisor)
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageMaster of Science (Department of Animal Science.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 001396225, proquestno: MM94733, Theses scanned by UMI/ProQuest.

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