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Žemaitukų mitochondrinės DNR konrolinės sekos nustatytmas ir palyginimas skirtingose arklių veislėse / Zemaitukai mitochondrial DNA control region identify and genetic relatioships between some other breeds

The objective - To identify Zemaitukai mtDNA D-loop region and genetic relationships between Zemaitukai and some other breeds based on mtDNA sequence variation.
Methods - To perform a phylogenetic analysis of 10 Zemaitukai, mtDNA D-loop 49 horses in the 20 different horse breeds sequence were included from GenBank (http://www.ncbi.nlm.nih.gov/GenBank). DNA was extracted from hair roots using the DNEasy® Tissue Kit (Qiagen). 1,280 bp fragment of mtDNA D-loop were amplify out in PTC-100™ termocycler. Amplified products were sequenced on a LI-COR® 4200S-2 automated sequencer. Sequencher v 4.1.4. software package was used to generate the actual DNA sequence for each of the animals. Multiple alignments of sequences were performed with CLUSTAL X 1.8 (Thompson et al., 1997). The Neighbor-joining tree (Saitou and Nei, 1987) of mtDNA sequences was constructed from Jukes-Cantor distances, performed on the pairwise deletion using the MEGA software (Kumar et al., 1993).
Results –Within Zemaitukai breed 19 polymorphic sites were detected and 6 haplotypes. At the individual level 4 zemaitukai haplotypes were private to one individuals, 1 haplotype-between 2 individuals and 1 haplotype was shared between 4 individuals. On the Neighbor-joining tree showed one Zemaitukai haplotype closer genetic relationships to the Icelandic and to the Norwegian Fiord horse haplotypes. Zemaitukai breed formed a separate branch with the ancient DNA haplotype. The phylogenetic analysis reflects the presence... [to full text]

Identiferoai:union.ndltd.org:LABT_ETD/oai:elaba.lt:LT-eLABa-0001:E.02~2005~D_20050413_083701-74154
Date13 April 2005
CreatorsDraudvilaitė, Kristina
ContributorsKučinskienė, Jūratė, Jauškevičius, Algirdas, Miceikienė, Ilona, Januškevičienė, Gražina, Juozaitienė, Vida, Lithuanian Veterinary Academy
PublisherLithuanian Academic Libraries Network (LABT), Lithuanian Veterinary Academy
Source SetsLithuanian ETD submission system
LanguageLithuanian
Detected LanguageEnglish
TypeMaster thesis
Formatapplication/pdf
Sourcehttp://vddb.library.lt/obj/LT-eLABa-0001:E.02~2005~D_20050413_083701-74154
RightsUnrestricted

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