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Previous issue date: 2011-07-01 / The topical corneal application of antimitotic mitomycin-C (MMC) during refractive surgery is still characterized by a lack of standardization and considerable empirism. For this reason the creation of a system capable of reliable drug delivery represents a beneficial innovation for patients submitted to these procedures. Objective: Elaborate a new MMC delivery system during the transoperatory period of photorefractive keratectomy (PRK) followed by patent application. Methods: The project consists of an in vitro experimental study to create an MMC (0.02%) release system. The drug was impregnated in sterile Whatman? 41 paper filter discs with a diameter of 8 mm. After drying, the discs were applied to antibiogram plates seeded with Staphylococcus epidermidis (American Type Culture Collection ATCC 12228), followed by the addition of a drop of sterile water. At the end of 1 minute, the discs were removed and the plates incubated for 48 hours at 35oC. Mean drop volume in the collyrium flasks was measured using analytical balance weighing. The inhibition halo (mm) was correlated with the MMC impregnated into the disc. After completion of the invention design a patent application was lodged at the National Institute of Industrial Property. Results: The correspondence between MMC-produced inhibition halos indicated that a dose of 16?g was ideal for impregnating into the discs. The mean drop volume obtained from the collyrium flasks was 37.7 ?L. A minute after the application of one drop of balanced saline solution, the system released an adequate concentration for PRK surgery. Conclusion: A new MMC delivery system was created for transoperatory application in photorefractive keratectomy (PRK). Publication of the patent application (number PI 0704739-8) gives the authors exclusive intellectual property rights. The study was sponsored by Ophthalmos Ind?stria e Com?rcio de Produtos Farmac?uticos S.A. (S?o Paulo-SP, Brazil) and received the indispensable scientific contribution of researchers from the fields of Pharmacy, Medicine, Biology, Statistics and Law, characterizing the work as multidisciplinary, in accordance with norms established by the Postgraduate Health Sciences Program of the Federal University of Rio Grande do Norte (UFRN) / A aplica??o t?pica corneana do antimit?tico mitomicina C (MMC) durante as cirurgias refrativas ainda ? caracterizada por uma falta de padroniza??o e grande empirismo. Por isso, a cria??o de um sistema capaz de disponibilizar a droga de forma padronizada representa uma inova??o que vem beneficiar os pacientes submetidos ?queles procedimentos. Objetivo: Elaborar um novo sistema de aplica??o da MMC durante o per?odo transoperat?rio da cirurgia de ceratectomia fotorrefrativa (Photorefractive keratectomy- PRK) com posterior dep?sito do Pedido de Patente. M?todos: O projeto representou um estudo experimental in vitro para cria??o de um sistema de libera??o de MMC a 0,02%. A droga foi impregnada em discos est?reis de papel filtro do tipo Whatman? 41 com 8 mm de di?metro. Ap?s o processo de secagem, os discos foram aplicados em placas de antibiograma semeadas com Staphylococcus epidermidis (American Type Culture Collection ATCC 12228), recebendo em seguida uma gota de ?gua est?ril. Decorrido o per?odo de 1 minuto, os discos foram removidos e as placas incubadas por 48 horas a 35oC. O volume m?dio das gotas de frascos de col?rio foi medido pelo m?todo de pesagem em balan?a anal?tica. O halo de inibi??o, em mil?metros, foi correlacionado com a dose da MMC impregnada no disco. Com o delineamento da inven??o, procedeu-se o Dep?sito do Pedido de Patente no Instituto Nacional da Propriedade Industrial. Resultados: A correspond?ncia entre os halos de inibi??o produzidos pela MMC indicou a dose de 16?g como a ideal para ser impregnada nos discos. O volume m?dio de uma gota obtida dos frascos de col?rio foi 37,7 ?L. Com a aplica??o de uma gota de solu??o salina balanceada e ap?s o per?odo de um minuto, o sistema liberou uma concentra??o considerada adequada para a cirurgia de PRK. Conclus?o: Um novo sistema de aplica??o de MMC foi criado para sua aplica??o transoperat?ria na ceratectomia fotorrefrativa (PRK). A publica??o do Pedido de Patente sob o n?mero PI 0704739-8 outorga aos autores a prioridade exclusiva de sua Propriedade Intelectual. A pesquisa teve o patroc?nio da Ophthalmos Ind?stria e Com?rcio de Produtos Farmac?uticos S.A. (S?o Paulo-SP, Brasil) e a indispens?vel contribui??o cient?fica de pesquisadores das ?reas de Farm?cia, Medicina, Biologia, Estat?stica e Direito, revestindo o trabalho de um car?ter multidisciplinar, requisito inerente ?s exig?ncias do Programa de P?s-Gradua??o de Ci?ncias da Sa?de da Universidade Federal do Rio Grande do Norte (UFRN)
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13235 |
| Date | 01 July 2011 |
| Creators | Pinheiro, Francisco Irochima |
| Contributors | CPF:00190403691, http://lattes.cnpq.br/8841547160023098, Silva J?nior, Arn?bio Ant?nio, CPF:00840406452, http://lattes.cnpq.br/2593509584288129, Cavalcante, Jeancarlo Fernandes, CPF:70231346468, http://lattes.cnpq.br/8403344128848984, Oliveira, Anselmo Gomes de, CPF:59434643872, http://lattes.cnpq.br/9114495952533044, Mendon?a, Marcelo Bezerra de Melo de, CPF:93202261487, http://lattes.cnpq.br/0863161064825884, Or?fice, Fernando |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias da Sa?de, UFRN, BR, Ci?ncias da Sa?de |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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