Kratom is an herbal substance that produces opioid-like and stimulant-like effects. Kratom contains bioactive alkaloids that include mitragynine and 7-OH mitragynine. Both substances activate mu-opioid receptors as well as bind to adrenergic, dopaminergic, and serotonergic receptors, which may be responsible for the stimulant effects. There is no current approved use of kratom or kratom products by the USFDA, but it is currently being used by individuals for management of drug withdrawal, pain, fatigue, and mental health problems. Multiple serious but rare side-effects have been reported, including gastrointestinal, respiratory, psychiatric, cardiovascular issues. Thus, the USDEA considers it to be a Drug and Chemical of Concern and has warned the public against risks of Kratom use. As such, research on Kratom products is necessary to better understand risks and inform policy regarding regulation. Here, we sought to develop a method by which the pharmacologically active mitragynine and its active metabolite 7-OH mitragynine could be measured in various kratom products. The quantification of each utilized high pressure liquid chromatography with ultra-violet detection (HPLC-UV). An XBridge C18 column with 3.5 um particle size, 4.6 x 150 mm was used, and separation was achieved using a gradient elution with acetonitrile and 0.1% formic acid. The flow rate was 1 mL/min, and the oven temperature was set at 40oC. UV detection was at 254 nm. The 7-OH mitragynine peak was visible at 2.5 minutes and the mitragynine at 3.55 minutes. In conclusion, this method has potential to provide utility for detection and quantification of pharmacologically active compounds in kratom products.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:asrf-2183 |
| Date | 25 April 2023 |
| Creators | Cifelli, Kathryn, PharmD Candidate, Jones, Madison, Brown, Stacy, Ph.D., Pond, Brooks, Ph.D. |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Source | Appalachian Student Research Forum |
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