Underexplored environments such as soil samples continue to be an untapped source of bacterial strains with great potential to produce potent secondary metabolites for medicinal applications. As a result, these microorganisms represent a broad and yet unknown reservoir of new strains capable of producing these novel natural compounds. Secondary metabolites from microorganisms have been used in antibiotic production, chemotherapy, immunosuppressants, and various industrial applications. The current research primarily seeks to perform the isolation, purification, and characterization of secondary metabolites from a soil bacterium (Janthinobacterium lividum TAJX1901). To achieve these objectives, the soil bacteria was successfully cultured on rich media agar plates followed by liquid–liquid extraction using a solvent mixture of methanol and chloroform(3:1). Various purification methods were utilized, including flash column chromatography, preparative thin layer chromatography, centrifugation, and high-performance liquid chromatography (HPLC ) using different column types and elution methods. For structural elucidation, UV/Vis analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were employed. The extraction resulted in a dominant violet pigment soluble in methanol. Preliminary results reveal the presence of highly conjugated, polar, and aromatic compounds. This work is relevant in the current global search for novel compounds for tackling antibiotic-resistant organisms and treating other diseases and infections.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:asrf-2169 |
| Date | 25 April 2023 |
| Creators | Agbakpo, Andy E, Arije, Amonah T, Fox, Sean, Shilabin, Abbas G |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Source | Appalachian Student Research Forum |
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