SILVA, J. S. P. Estudo das caracter?sticas f?sico-qu?micas e biol?gicas pela
ades?o de osteoblastos em superf?cies de tit?nio modificadas pela nitreta??o
em plasma. 2008. 119 f. Tese (Doutorado) - Faculdade de Medicina, Universidade de S?o
Paulo. S?o Paulo, 2008. / Submitted by CECILIA SANTOS (cecilia@bczm.ufrn.br) on 2010-09-29T13:32:45Z
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license_rdf: 20464 bytes, checksum: d540d98f4c56c073d1d99228e27d53ca (MD5) / PURPOSE: The aim of this study was to evaluated the physico-chemical
properties of different titanium surfaces modified by means of low
temperature plasma nitridind on rat osteoblast cell adhesion and proliferation.
METHODS: Pure Titanium discs grade II was submitted to three different
surface preparations (polishing, glowglow discharge plasma nitriding in planar and
cathodic cage configurations). Surface parameters as roughness, wettability
and chemichal composition was determined to compare influency of gas
mixture on the modified surface material properties. Cellular morphology was
observed by scanning electron microscopy.To evaluate the effect of the
surface on cellular response, osteoblast cells (MC3T3) adhesion and
proliferation was quantified and data analised by Kruskal-Wallis and
Friedman statistical tests. RESULTS: plasma nitriding discs shows rougher
surfaces( p<0,02) in cathodic cage configuration andlower contact angle
values. MC3T3 cells attached on rough surfaces produced by cathodic cage
configuration was statistically significant p<0,05 compared to polished discs.
CONCLUSIONS: Glow discharge plasma nitriding improve titanium surface
roughness and wettability. MC3T3 cell adhesion behavior is related to substrate chemical composition and topography. Resumo: INTRODU??O: Superf?cies de tit?nio modificadas por diferentes m?todos
foram estudadas com base nos par?metros f?sicos e qu?micos de
caracteriza??o superficial e sua influ?ncia no comportamento de c?lulas pr?osteobl?sticas
(MC3T3) in vitro. M?TODOS: Discos de tit?nio
comercialmente puro grau II foram submetidos a tr?s m?todos de
modifica??o de superf?cie (polimento, nitretados em plasma em configura??o
planar e gaiola cat?dica). As diferentes superf?cies foram caracterizadas
para observar o efeito do processamento na estrutura da camada superficial,
na rugosidade e molhabilidade. Ensaios de ades?o e prolifera??o celular
usando linhagens de c?lulas pr?-osteobl?sticas MC3T3 foram realizados
para avaliar o efeito das novas superf?cies no comportamento celular in vitro.
RESULTADOS: Os resultados demonstraram que a nitreta??o em plasma
na configura??o de gaiola cat?dica produz superf?cies mais rugosas (p<0,02)
e com menores ?ngulos de contato com a ?gua. CONCLUS?ES: A ades?o
celular ? maior nas superf?cies mais rugosas do que nas superf?cies polidas
(p<0,05) e reagem de modo diferente a composi??o qu?mica do substrato e ? topografia da superf?cie
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:1/3075 |
| Date | January 2008 |
| Creators | Silva, Jos? Sandro Pereira da |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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