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Detection of Epstein-Barr virus related gene products and tumour gene products in nasopharyngeal carcinoma.

by Shik Yuen Lo. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves 102-124). / Abstract / List of Illustrations / List of Tables / Acknowledgements / Chapter 1. --- Introduction --- p.1 / Chapter 2. --- Literature Review / Chapter 2.1 --- Anatomy of the Human Nasopharynx --- p.4 / Chapter 2.2 --- Histology of the Human Nasopharynx --- p.6 / Chapter 2.3 --- Intra-epithelial Lesions of the Nasopharyngeal Epithelium --- p.10 / Chapter A. --- Hyperplasia / Chapter B. --- Metaplasia / Chapter C. --- Koilocytes / Chapter D. --- Nasopharyngeal intra-epithelial neoplasia / Chapter 2.4 --- Nasopharyngeal Carcinoma --- p.19 / Chapter A. --- Histopathological classification of NPG / Chapter B. --- Epidemiology / Chapter C. --- Etiological factors / Chapter 2.5 --- Epstein-Barr Virus and Nasopharyngeal Carcinoma --- p.27 / Chapter A. --- Serological / Chapter B. --- EBV genome in NPC / Chapter C. --- EBV encoded latent gene products / Chapter 2.6 --- Cancer Genes in Nasopharyngeal Carcinoma --- p.28 / Chapter A. --- "Tumours suppressor Gene, p53" / Chapter B. --- "Oncogenes, c-myc, ras and bcl-2" / Chapter 2.7 --- Immunohistochemical methods --- p.33 / Chapter A. --- Avidin-Biotin Complex method (ABC) / Chapter B. --- Alkaline phosphotase Anti-alkaline phosphotase method (APAAP) / Chapter C. --- Unmasking of antigens / Chapter 2.8 --- Techniques in ISH --- p.40 / Chapter 3. --- Material and Methods --- p.42 / Chapter 3.1 --- Tissue Samples --- p.42 / Chapter A. --- "Samples for ras, c-myc and p53 studies" / Chapter B. --- Samples for LMP-1 study / Chapter C. --- Samples for bcl-2 study / Chapter D. --- Samples for EBER-RNAs study / Chapter 3.2 --- Monoclonal Antibodies --- p.47 / Chapter 3.3 --- Tissue Processing --- p.49 / Chapter A. --- Tissue processing for formalin fixed tissue / Chapter B. --- Tissue processing for frozen section / Chapter 3.4 --- IHC Techniques --- p.50 / Chapter A. --- Pretreatment of Laboratory Wares / Chapter B. --- Determination of optimum dilution and incubation time for p53antibody / Chapter C. --- Determination of optimum dilution and incubation time for bcl-2 and LMP-1 antibodies / Chapter D. --- Determination of optimum dilution and incubation time for c-myc and ras / Chapter E. --- "Detection of p53, c-myc and ras by ABC method" / Chapter F. --- Detection of bcl-2 and LMP-1 by APAAP method / Chapter 3.6 --- ISH --- p.57 / Chapter A. --- Pretreatment of laboratory wares / Chapter B. --- FITC conjugated EBER oligonucleotide probe / Chapter C. --- Determination of PK dilution for paraffin section / Chapter D. --- Determination of PK dilution for frozen section / Chapter E. --- Determination of the choice of fixative for frozen section / Chapter F. --- Detection of EBER-RNAs by ISH method / Chapter 3.7 --- Statistical analysis --- p.62 / Chapter A. --- p53 / Chapter B. --- c-myc and ras / Chapter 4. --- Results --- p.63 / Chapter A. --- ras / Chapter B. --- c-myc / Chapter C. --- p53 / Chapter D. --- LMP-1 / Chapter E. --- Bcl-2 / Chapter F. --- EBER-RNAs / Chapter 5. --- Discussion --- p.86 / Chapter 6. --- Conclusion and Summary --- p.97 / Appendix --- p.99 / Reference --- p.102

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_320702
Date January 1995
ContributorsLo, Shik Yuen., Chinese University of Hong Kong Graduate School. Division of Pathological Sciences.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, 124 leaves : ill. (some mounted col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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