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Immunological detection and the binding protein of Nervous Necrosis Virus infecting Epinephelus malabaricus

Nervous necrosis virus of Epinephelus malabaricus (MGNNV) belongs to the genus of Betanodavirus that causes vacuolating encephalopathy and retinopathy and viral nervous necrosis. A method to purify MGNNV was proposed. The cellular components of SSN-1 reprent few viral receptors for Betanodavirus. Several cellular virus binding proteins (VBPs) were detected by employing the technique of immobilizing virus on nitrocellulose. The least VBPs were found in SSN-1 cell lysate that was treated with proteinase K. The approaches used receptor antagonists to identify the cell receptor. The antagonists are able to block the viral binding and thus potentially directly against the receptor. The results implied that the receptor of serotonin 5-HT1A or £\2 adrenergic may act as the receptor of MGNNV.

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0813102-102827
Date13 August 2002
CreatorsLe, Wan-Chi
ContributorsChan-Shing Lin, Chi-Hsin Hsu, Jong-Kang Liu
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageCholon
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0813102-102827
Rightsnot_available, Copyright information available at source archive

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